Entwicklung eines kontaktlosen Verbrennungsmodells zur Mikrozirkulationsanalyse und perkutanen Thrombozytendarstellung

$\bf Ziel:$ war es ein kontaktloses Verbrennungsmodell zu entwickeln welches die Analyse der Mikrozirkulation nach Verbrennung und eine Untersuchung von Thrombozyten erlaubt. $\bf Methode:$ Wir entwickelten ein kontaktloses Verbrennungsmodell am Ohr der haarlosen Maus zur Analyse der Mikrozirkulation nach Verbrennung. Thrombozyten wurden isoliert, angefärbt, in einer FACS-Analyse untersucht und anschließend im Versuchstier fluoreszenzmikroskopisch untersucht. $\bf Ergebnis:$ Mit dem Modell ließen sich reproduzierbare tiefgradige Verbrennungen erzeugen. Entzündungsreaktion und Ödembildung waren an Tag 1 nach Verbrennung maximal, das nicht perfundierte Areal nahm kontinuierlich ab. Eine fluoreszenzmikroskopische Untersuchung der Thrombozyten im Versuchstier war nicht möglich. $\bf Diskussion:$ Das Modell kann als Grundlage für weitere Studien genutzt werden. Die wesentlichen Prozesse in der Wunddynamik finden in einem engeren zeitlichen Rahmen statt als bisher angenommen

Additional file 1: of Enzymatic debridement for the treatment of severely burned upper extremities – early single center experiences

Raw data. (XLSX 31 kb

Low-energy extracorporeal shockwave therapy (ESWT) improves metaphyseal fracture healing in an osteoporotic rat model

<div><p>Purpose</p><p>As result of the current demographic changes, osteoporosis and osteoporotic fractures are becoming an increasing social and economic burden. In this experimental study, extracorporeal shock wave therapy (ESWT), was evaluated as a treatment option for the improvement of osteoporotic fracture healing.</p><p>Methods</p><p>A well-established fracture model in the metaphyseal tibia in the osteoporotic rat was used. 132 animals were divided into 11 groups, with 12 animals each, consisting of one sham-operated group and 10 ovariectomized (osteoporotic) groups, of which 9 received ESWT treatment. Different energy flux intensities (0.15 mJ/mm², 0.35 mJ/mm², or 0.55 mJ/mm²) as well as different numbers of ESWT applications (once, three times, or five times throughout the 35-day healing period) were applied to the osteoporotic fractures. Fracture healing was investigated quantitatively and qualitatively using micro-CT imaging, quantitative real-time polymerase chain reaction (qRT-PCR) analysis, histomorphometric analysis and biomechanical analysis.</p><p>Results</p><p>The results of this study show a qualitative and quantitative improvement in the osteoporotic fracture healing under low-energy (energy flux intensity: 0,15 mJ/mm²) ESWT and with fewer treatment applications per healing period.</p><p>Conclusion</p><p>In conclusion, low-energy ESWT seems to exhibit a beneficial effect on the healing of osteoporotic fractures, leading to improved biomechanical properties, enhanced callus-quantity and -quality, and an increase in the expression of bone specific transcription factors. The results suggest that low-energy ESWT, as main treatment or as adjunctive treatment in addition to a surgical intervention, may prove to be an effective, simple to use, and cost-efficient option for the qualitative and quantitative improvement of osteoporotic fracture healing.</p></div

Biomechanical analysis results.

<p>Biomechanical analysis results regarding the average A) stiffness in N/mm and B) yield load in N. Both are shown against the different treatment groups (number of treatment(s) and applied energy flux intensity (mJ/mm²)). Values are shown as means ± standard error of the mean (SEM). Adjusted p-values were considered statistically significant at p < 0.05 (One-way analysis of variance (SPSS Statistics Version 23; IBM Corp., New York, NY, USA), Welch-Test adjusted, and Bonferroni post hoc test for stiffness; Two- way analysis of variance ((SPSS Statistics Version 23; IBM Corp., New York, NY, USA) and Bonferroni post hoc test for the other parameters). * p < 0.05 vs. 3 x 0.55 mJ/mm². N: Newton, mm: milli-meter, mJ: milli-Joule.</p

Gene expression analysis of osteoblast and osteoclast specific transcripts.

<p>Gene expression analysis through qRT-PCR of the osteoblast and osteoclast markers with regards to the respective treatment group (number of treatment(s) and energy flux intensity (mJ/mm²)) and shown in relative expression units. Portrayed values are shown as means ± standard error of the mean (SEM). Adjusted p-values were considered statistically significant at p < 0.05 (One-way analysis of variance (SPSS Statistics Version 23; IBM Corp., New York, NY, USA), Welch-Test adjusted, and Bonferroni post hoc test). Please see Supplement 2 for details on the numerous significances. qRT-PCR: Quantitative real-time Polymerase Chain Reaction, Coll1α1: Collagen 1-alpha-1, ERα: Estrogen Receptor-α, IGF-1: Insulin-like Growth Factor 1, OC: Osteocalcin, TRAP: Tartrate-resistant Acid Phosphatase, mJ: milli-Joule, mm: millimeter.</p

Specific primer sequences used for the quantitative real-time polymerase chain reaction (qRT-PCR) amplification.

<p>Specific primer sequences used for the quantitative real-time polymerase chain reaction (qRT-PCR) amplification.</p

Fracture osteosynthesis of the tibia.

<p>Completed osteosynthesis of the tibia with a 5-hole mini Y-plate and 4 screws, with the plate spanning the fracture gap located in the metaphyseal area of the tibia.</p