Intersect of significant miRs from the adenocarcinoma cases when using non-paired (DESeq) and paired (edgeR) analysis approach.

<p>Adjusted for multiple testing using Benjamini and Hochberg, false discovery rate (FDR) < 0.1. Logarithmic fold change (FC) relative to normal mucosa and FDR from paired analysis using edgeR. miRs also significant in the analysis of the neuroendocrine tumor (NET) is indicated.</p

Figure 2

<p><b>Schematic illustration of statistical approach.</b> Panels A and C show approach using non-paired statistics and the DESeq tool. Panel B shows approach using paired statistics and the edgeR tool. See text for further details.</p

<i>NFKB1</i> mRNA levels in morphologicallynon-affected and affected tissues normalised to the <i>β-actin</i> mRNA level.

<p>NS = not significant.</p>a<p>P-value for the comparison to the expression levels in tissue from healthy individuals using Kruskal Wallis and Dunńs Multiple Comparison test.</p>b<p>P-value for the comparison of the expression levels in normal and affected tissue from the same individual using Paired Student’s t-test.</p

Clinical and histopathological characteristics of patients in the study.

<p>Clinical and histopathological characteristics of patients in the study.</p

Characteristics of study participants.

1<p>There were significantly more males in the group with mild dysplasia and colorectal cancer compared to the group of healthy individuals (p<0.001, Chi squared test).</p>2<p>The age is significant higher among patients with CRC compared to the three other groups (Kruskal-Wallis and Dunn's Multiple Comparison test, p<0.001).</p>3<p>Normal endoscopy.</p

Venn diagram shows the number of significant miRs identified using the non-paired (DESeq) and paired (edgeR) analysis approach.

<p>Venn diagram shows the number of significant miRs identified using the non-paired (DESeq) and paired (edgeR) analysis approach.</p

<i>ABCB1</i> mRNA levels in morphologically normal and affected tissues normalised to the <i>β-actin</i> mRNA level.

<p><i>*P-</i>value <0.05, **<i>P-</i>value <0.01, ***<i>P-</i>value <0.001.</p

<i>NFKB1</i> mRNA levels in morphologically normal and affected tissues normalised to the <i>β-actin</i> mRNA level.

<p>***<i>P-</i>value <0.001.</p

Read classification as predicted by miRanalyzer and miRBase.

<p>Panel A with percentage of sequencing reads mapped to mature miRs (black) of the total reads per experiment. Panel B with number of mature miRs identified per sequencing experiment. The total number of mature human miRs in miRBase release 16 (n = 1212) is included as reference.</p

<i>ABCG2</i> mRNA levels x 10⁷ in intestinal tissues normalised to 18S RNA levels

<p>^a P-values for comparison of the expression levels in tissue from healthy individuals adjusted for age and gender.</p><p>^b P value for the comparison of the expression levels in morphologically unaffected and affected tissue from the same individual using Paired Student’s t-test. Matching samples were available from 66 cases with mild-moderate dysplasia, 11 cases with severe dysplasia, and 80 and 83 CRC cases (distant unaffected tissue, and adjacent unaffected tissue, respectively.</p><p><i>ABCG2</i> mRNA levels x 10⁷ in intestinal tissues normalised to 18S RNA levels</p