8 research outputs found
Body Site Is a More Determinant Factor than Human Population Diversity in the Healthy Skin Microbiome
<div><p>We studied skin microbiota present in three skin sites (forearm, axilla, scalp) in men from six ethnic groups living in New York City. <b>Methods.</b> Samples were obtained at baseline and after four days following use of neutral soap and stopping regular hygiene products, including shampoos and deodorants. DNA was extracted using the MoBio Power Lyzer kit and 16S rRNA gene sequences determined on the IIlumina MiSeq platform, using QIIME for analysis. <b>Results.</b> Our analysis confirmed skin swabbing as a useful method for sampling different areas of the skin because DNA concentrations and number of sequences obtained across subject libraries were similar. We confirmed that skin location was the main factor determining the composition of bacterial communities. Alpha diversity, expressed as number of species observed, was greater in arm than on scalp or axilla in all studied groups. We observed an unexpected increase in α-diversity on arm, with similar tendency on scalp, in the South Asian group after subjects stopped using their regular shampoos and deodorants. Significant differences at phylum and genus levels were observed between subjects of the different ethnic origins at all skin sites. <b>Conclusions.</b> We conclude that ethnicity and particular soap and shampoo practices are secondary factors compared to the ecological zone of the human body in determining cutaneous microbiota composition.</p></div
Comparison of microbiota differences by ethnic group, analyzed by LEfSe using baseline samples.
<p>Taxa with relative abundance ≥ 0.1% present in at least one sample in each location were included. The cladograms indicate the phylogenetic distribution of the microbial lineages associated with ethnic group, with lineages with Linear Discriminant Analysis (LDA) score ≥2.0 displayed. Significance differences for each ethnic group of the most abundant class are indicated by color, as indicated in the key. Each node’s diameter is proportional to the taxon’s abundance. The strategy of multiclass analysis is non-strict (≥ one class differential). Nodes represent phylogenetic levels from domain to genus (from inside out) (AA, n = 18 subjects; AC, n = 11; CA, n = 16; EA, n = 25; LA, n = 19; SA, n = 20).</p
Taxonomic analysis of cutaneous microbiota from 110 subjects in six different subject groups.
<p><b>Panel A.</b> By site (arm, axilla, scalp) at the phylum level. A total of 41 phyla were found. The sequences for the dominant 9 phyla (>0.1% in any group) accounted for >99.7% of total sequences in all ethnic groups. Baseline: Samples collected before special soap wash used. Follow-up: Samples collected after special soap wash used. <b>Panel B:</b> Genus level. A total 726 genera were detected; only predominant genera (Mean>0.01%) are shown.</p
Comparison of diversity of the cutaneous microbiota over time, by ethnic group.
<p><b>Panel A.</b> Rarefaction analysis of cutaneous microbiota in relation to sampling time in samples from arms (Brown), axilla (Black) and scalp (Yellow), African-American (AA, n = 108 samples); African-Continental (AC, n = 66); Caucasian-American (CA, n = 96); East-Asian (EA, n = 150); Latin-American (LA, n = 114); South-Asian (SA, n = 96). Rarefaction analysis represented by phylogenetic distance. The solid and dashed lines indicate samples collected before (T1) and after (T2) a special soap wash was used, respectively. Only a significant difference between time-points was found for the SA group. <b>Panel B.</b> Intra- and inter-group beta-diversity over time. Mean (±SD) pairwise unweighted UniFrac distances are shown. The ethnic groups are colored as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0151990#pone.0151990.g001" target="_blank">Fig 1</a>. Significance was determined by one-way ANOVA with the Tukey method for correction for multiple comparisons (*<0.05; **<0.01; ***<0.001; ****<0.0001).</p
Cutaneous microbial diversity in 645 samples from 110 subjects, based on skin location and beta diversity analysis over time, by ethnic group.
<p>The sequence depth is 3200/sample. <b>Panel A.</b> PCoA of clustering by sample location, based on unweighted pairwise UniFrac distances. The skin locations were Scalp [S (n = 215 samples)]; Arm [Ar (n = 215)]; Axilla [Ax (n = 215)]. <b>Panel B</b>. Analysis by ethnic group, including African-American [AA (n = 18 subjects/site)], East Asian [EA (n = 25)], Caucasian-American [CA (n = 16)], Latin American [LA (n = 16)], African-Continental [AC (n = 11)], and South Asian [SA (n = 19)]. PCoA of clustering of skin samples combining the three cutaneous sites, and the two time points; 1 = baseline and 2 = follow-up. Ellipses generally describe: axilla (black), scalp (yellow), and Arm (brown). <b>Key to explain sites sampled and ethnic groups in all figures:</b> The skin locations are colored as follows: yellow: Scalp (S); Brown: Arm (Ar); Black: Axilla (Ax). Ethnic groups are colored as: Orange: South Asian (SA); Light blue: Latin-American (LA); Purple: East Asian (EA); Red: African-American (AA); Blue: Caucasian-American (CA); Green: African-Continental (AC).</p
Analysis of cutaneous microbiota by ethnicity.
<p>The results are from 6 ethnic groups, including Caucasian-American (n = 20/site), African-American (n = 18/site), American-Continental (n = 11/site), Latin American (n = 16/site) East Asian (n = 25/site), and South Asian (n = 20/site). <b>Panel A.</b> Alpha diversity represented by phylogenetic distances. Arm, significant differences were found between EA vs. LA and AA; and SA vs. LA. Axilla, between EA vs. AA, LA and CA. Scalp, between EA vs. AC and CA; AA vs. AC and CA. The ethnic groups are colored as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0151990#pone.0151990.g001" target="_blank">Fig 1</a>. <b>Panel B.</b> PCoA of clustering by ethnic group, based on unweighted pairwise Unifrac distances. The circles represent the distribution of the East Asian samples. <b>Panel C.</b> Intra- and inter-group beta-diversity analysis on skin location at baseline. Mean (±SD) pairwise unweighted UniFrac distances shown. The * symbol indicate that the intra-group beta diversity was significantly different (p<0.001).</p
Additional file 3: of Exome-chip meta-analysis identifies novel loci associated with cardiac conduction, including ADAMTS6
Video S1. (Quicktime) Video to illustrate the DORV phenotype finding in an Adamts6 mutant heart. (MOV 1983 kb
Additional file 2: of Exome-chip meta-analysis identifies novel loci associated with cardiac conduction, including ADAMTS6
Figure S1. Manhattan plot for European and African-American ancestry single variant analysis. Figure S2. Quantile-quantile plot for European and African-American ancestry single variant analysis. Figure S3. Manhattan plot for EA single variant analysis. Figure S4. QQ plot for EA single variant analysis. Figure S5. Manhattan plot for AA single variant analysis. Figure S6. Quantile-quantile plot for AA single variant analysis. Figure S7. Miami plot European and African-American ancestry sex-stratified single variant analysis. Figure S8. Quantile-quantile plots for European and African-American ancestry sex-stratified single variant analyses. Figure S9. Normal morphology of adult Adamts6 heterozygous hearts. (DOCX 4290 kb