1 research outputs found
Non-Neurotoxic Nanodiamond Probes for Intraneuronal Temperature Mapping
Optical biomarkers have been used
extensively for intracellular
imaging with high spatial and temporal resolution. Extending the modality
of these probes is a key driver in cell biology. In recent years,
the nitrogen-vacancy (NV) center in nanodiamond has emerged as a promising
candidate for bioimaging and biosensing with low cytotoxicity and
stable photoluminescence. Here we study the electrophysiological effects
of this quantum probe in primary cortical neurons. Multielectrode
array recordings across five replicate studies showed no statistically
significant difference in 25 network parameters when nanodiamonds
are added at varying concentrations over various time periods, 12–36
h. The physiological validation motivates the second part of the study,
which demonstrates how the quantum properties of these biomarkers
can be used to report intracellular information beyond their location
and movement. Using the optically detected magnetic resonance from
the nitrogen-vacancy defects within the nanodiamonds we demonstrate
enhanced signal-to-noise imaging and temperature mapping from thousands
of nanodiamond probes simultaneously. This work establishes nanodiamonds
as viable multifunctional intraneuronal sensors with nanoscale resolution,
which may ultimately be used to detect magnetic and electrical activity
at the membrane level in excitable cellular systems