417 research outputs found
Status of the EDELWEISS-II experiment
EDELWEISS is a direct dark matter search experiment situated in the low
radioactivity environment of the Modane Underground Laboratory. The experiment
uses Ge detectors at very low temperature in order to identify eventual rare
nuclear recoils induced by elastic scattering of WIMPs from our Galactic halo.
We present results of the commissioning of the second phase of the experiment,
involving more than 7 kg of Ge, that has been completed in 2007. We describe
two new types of detectors with active rejection of events due to surface
contamination. This active rejection is required in order to achieve the
physics goals of 10-8 pb cross-section measurement for the current phase
Cryptococcal transmigration across a model brain blood-barrier: Evidence of the Trojan horse mechanism and differences between Cryptococcus neoformans var. grubii strain H99 and Cryptococcus gattii strain R265
© 2015 Institut Pasteur. Cryptococcus neoformans (. Cn) and Cryptococcus gattii (Cg) cause neurological disease and cross the BBB as free cells or in mononuclear phagocytes via the Trojan horse mechanism, although evidence for the latter is indirect. There is emerging evidence that Cn and the North American outbreak Cg strain (R265) more commonly cause neurological and lung disease, respectively. We have employed a widely validated in vitro model of the BBB, which utilizes the hCMEC/D3 cell line derived from human brain endothelial cells (HBEC) and the human macrophage-like cell line, THP-1, to investigate whether transport of dual fluorescence-labelled Cn and Cg across the BBB occurs within macrophages. We showed that phagocytosis of Cn by non-interferon (IFN)-γ stimulated THP-1 cells was higher than that of Cg. Although Cn and Cg-loaded THP-1 bound similarly to TNF-activated HBECs under shear stress, more Cn-loaded macrophages were transported across an intact HBEC monolayer, consistent with the predilection of Cn for CNS infection. Furthermore, Cn exhibited a higher rate of expulsion from transmigrated THP-1 compared with Cg. Our results therefore provide further evidence for transmigration of both Cn and Cg via the Trojan horse mechanism and a potential explanation for the predilection of Cn to cause CNS infection
Exploring experimental cerebral malaria pathogenesis through the characterisation of host-derived plasma microparticle protein content
© 2016 The Author(s). Cerebral malaria (CM) is a severe complication of Plasmodium falciparum infection responsible for thousands of deaths in children in sub-Saharan Africa. CM pathogenesis remains incompletely understood but a number of effectors have been proposed, including plasma microparticles (MP). MP numbers are increased in CM patients' circulation and, in the mouse model, they can be localised within inflamed vessels, suggesting their involvement in vascular damage. In the present work we define, for the first time, the protein cargo of MP during experimental cerebral malaria (ECM) with the overarching hypothesis that this characterisation could help understand CM pathogenesis. Using qualitative and quantitative high-throughput proteomics we compared MP proteins from non-infected and P. berghei ANKA-infected mice. More than 360 proteins were identified, 60 of which were differentially abundant, as determined by quantitative comparison using TMT TM isobaric labelling. Network analyses showed that ECM MP carry proteins implicated in molecular mechanisms relevant to CM pathogenesis, including endothelial activation. Among these proteins, the strict association of carbonic anhydrase I and S100A8 with ECM was verified by western blot on MP from DBA/1 and C57BL/6 mice. These results demonstrate that MP protein cargo represents a novel ECM pathogenic trait to consider in the understanding of CM pathogenesis
Proceedings of the third French-Ukrainian workshop on the instrumentation developments for HEP
The reports collected in these proceedings have been presented in the third
French-Ukrainian workshop on the instrumentation developments for high-energy
physics held at LAL, Orsay on October 15-16. The workshop was conducted in the
scope of the IDEATE International Associated Laboratory (LIA). Joint
developments between French and Ukrainian laboratories and universities as well
as new proposals have been discussed. The main topics of the papers presented
in the Proceedings are developments for accelerator and beam monitoring,
detector developments, joint developments for large-scale high-energy and
astroparticle physics projects, medical applications.Comment: 3rd French-Ukrainian workshop on the instrumentation developments for
High Energy Physics, October 15-16, 2015, LAL, Orsay, France, 94 page
Final results of the EDELWEISS-II WIMP search using a 4-kg array of cryogenic germanium detectors with interleaved electrodes
The EDELWEISS-II collaboration has completed a direct search for WIMP dark
matter with an array of ten 400-g cryogenic germanium detectors in operation at
the Laboratoire Souterrain de Modane. The combined use of thermal phonon
sensors and charge collection electrodes with an interleaved geometry enables
the efficient rejection of gamma-induced radioactivity as well as near-surface
interactions. A total effective exposure of 384 kg.d has been achieved, mostly
coming from fourteen months of continuous operation. Five nuclear recoil
candidates are observed above 20 keV, while the estimated background is 3.0
events. The result is interpreted in terms of limits on the cross-section of
spin-independent interactions of WIMPs and nucleons. A cross-section of
4.4x10^-8 pb is excluded at 90%CL for a WIMP mass of 85 GeV. New constraints
are also set on models where the WIMP-nucleon scattering is inelastic.Comment: 23 pages, 5 figures; matches published versio
A search for low-mass WIMPs with EDELWEISS-II heat-and-ionization detectors
We report on a search for low-energy (E < 20 keV) WIMP-induced nuclear
recoils using data collected in 2009 - 2010 by EDELWEISS from four germanium
detectors equipped with thermal sensors and an electrode design (ID) which
allows to efficiently reject several sources of background. The data indicate
no evidence for an exponential distribution of low-energy nuclear recoils that
could be attributed to WIMP elastic scattering after an exposure of 113 kg.d.
For WIMPs of mass 10 GeV, the observation of one event in the WIMP search
region results in a 90% CL limit of 1.0x10^-5 pb on the spin-independent
WIMP-nucleon scattering cross-section, which constrains the parameter space
associated with the findings reported by the CoGeNT, DAMA and CRESST
experiments.Comment: PRD rapid communication accepte
Muon-induced background in the EDELWEISS dark matter search
A dedicated analysis of the muon-induced background in the EDELWEISS dark
matter search has been performed on a data set acquired in 2009 and 2010. The
total muon flux underground in the Laboratoire Souterrain de Modane (LSM) was
measured to be \,muons/m/d. The
modular design of the muon-veto system allows the reconstruction of the muon
trajectory and hence the determination of the angular dependent muon flux in
LSM. The results are in good agreement with both MC simulations and earlier
measurements. Synchronization of the muon-veto system with the phonon and
ionization signals of the Ge detector array allowed identification of
muon-induced events. Rates for all muon-induced events and of WIMP-like events were extracted. After
vetoing, the remaining rate of accepted muon-induced neutrons in the
EDELWEISS-II dark matter search was determined to be at 90%\,C.L. Based on
these results, the muon-induced background expectation for an anticipated
exposure of 3000\,\kgd\ for EDELWEISS-3 is
events.Comment: 21 pages, 16 figures, Accepted for publication in Astropart. Phy
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