1 research outputs found
Metabolomics and Lipidomics Profiling of a Combined Mitochondrial Plus Endoplasmic Reticulum Fraction of Human Fibroblasts: A Robust Tool for Clinical Studies
Mitochondria
and endoplasmic reticulum (ER) are physically and
functionally connected. This close interaction, via mitochondria-associated
membranes, is increasingly explored and supports the importance of
studying these two organelles as a whole. Metabolomics and lipidomics
are powerful approaches for the exploration of metabolic pathways
that may be useful to provide deeper information on these organellesā
functions, dysfunctions, and interactions. We developed a quick and
simple experimental procedure for the purification of a mitochondria-ER
fraction from human fibroblasts. We applied combined metabolomics
and lipidomics analyses by mass spectrometry with excellent reproducibility.
Seventy-two metabolites and 418 complex lipids were detected with
a mean coefficient of variation around 12%, among which many were
specific to the mitochondrial metabolism. Thus this strategy based
on robust mitochondria-ER extraction and āomicsā combination
will be useful for investigating the pathophysiology of complex diseases