Additional file 3: of Physical basis of the ‘magnification rule’ for standardized Immunohistochemical scoring of HER2 in breast and gastric cancer

Figure S3. Scatter-plot of HER2 DAB-precipitates width and color intensity. For scoring intensities 1+ and 2+ (grey), width and intensity show a linear correlation (r = 0.73, dashed lined). Scoring category 3+ shows saturated intensity (n = 1200 measurements in 40 cases per scoring category). (JPEG 585 kb

Additional file 1: of Physical basis of the ‘magnification rule’ for standardized Immunohistochemical scoring of HER2 in breast and gastric cancer

Figure S1. Example photomicrographs of HER2-IHC. Images depict scoring categories 1+, 2+ and 3+ at magnifications reflecting different microscope objectives (2.5× - 63×. Inserts: Magnified details, 4× additional magnification). Note that the linear DAB-precipitates in categories 1+ and 2+ are not perceivable at low power magnification (2.5×, 5×). (TIFF 47314 kb