Cell migration and invasion ability were inhibited by increasing concentrations of simvastatin.

<p>(A) Treatment with 0, 8 and 16 µm of simvastatin showed inhibited migration and invasion of A498 cells. (B) The number of A498 cells that successfully migrated and invaded was counted. (C) Migration and invasion of 786-O cells was inhibited after treatment with different concentrations of simvastatin. (D) The decreased number of 786-O cells indicated the great inhibitory effect of simvastatin on cell mobility. Data is presented as mean ± SD of three independent experiments. * <i>P</i><0.05, ** <i>P</i><0.01.</p

Simvastatin induced dose-dependent apoptosis in A498 and 786-O cells.

<p>(A) A498 and (B) 786-O cells were treated with simvastatin (0, 8 and 16 µM) for 48 hours and stained with FITC-annexinV and PI. The percentage of surviving cells was shown in the lower left quadrant; the percentage of early stage of apoptosis and late stage of apoptosis cells were shown in the lower right and upper right quadrants, respectively. (C, D) The quantification of apoptosis induced by simvastatin was calculated. Data is presented as mean ± SD of three independent experiments. * P<0.05, ** P<0.01.</p

Anti-tumor effects of simvastatin on A498 tumor xenograft were associated with the inhibition of AKT, ERK and STAT3 activity.

<p>Western blotting analysis for the phosphorylated AKT, ERK and STAT3 levels in tumor xenograft collected from nude mice treated with or without simvastatin. Quantitation of p-AKT/AKT, p-ERK/ERK and p-STAT3/STAT3 ratio was determined by densitometry analysis. *P<0.05 compared with control.</p

Simvastatin depressed proliferation and metastasis of A498 cells that were transfected with AKT or ERK siRNA compared with the control cells (Mock).

<p>(A, B) A498 cells were transfected and treated with simvastatin (8 µM), and the levels of AKT and ERK were analyzed by western blotting with GAPDH as a control. After transfected with AKT or ERK siRNA, A498 cells were incubated in the absence or presence of simvastatin (8 µM) for 48 h. The cell viability was measured by MTT assay (C, D), and cell migration and invasion was measured by transwell assay (E, F). * p<0.05 or ** p<0.01, compared with the untreated cells (Mock). # p<0.05 or ## p<0.01, compared with the cells transfected with AKT or ERK siRNA.</p

Effects of simvastatin on the protein levels of Bcl-2, Bax, caspase 3, PARP, mTOR, AKT, and ERK in A498 and 786-O cells.

<p>(A) A498 and 786-O cells were assayed for Bcl-2, Bax, full length caspase 3 and cleaved caspase 3, full length PARP and cleaved PARP by western blotting analysis with GAPDH as a control. (B) Bax/Bcl-2 ratios of A498 and 786-O cells. The densitometry value of each band was determined with ImageJ. Data was presented as mean ± SD of three independent experiments. * P<0.05, ** P<0.01. (C–H) The levels of mTOR, AKT, ERK and their phosphorylated forms were analyzed by western blotting. Quantitation of the p-mTOR/mTOR, p-AKT/AKT and p-ERK/ERK ratio was determined by densitometry analysis.</p

Simvastatin inhibited IL-6 induced proliferation, migration and invasion of A498 cells via inhibition of JAK2/STAT3 pathway.

<p>(A, B) A498 cells were treated with IL-6 (10 ng/ml) for 48 h, and cell vitality and motility was estimated using the MTT and transwell assay respectively. Each bar represents the mean±SD of three independent experiments. *p<0.05 or ** p<0.01, compared with cells treated without IL-6. (C–H) Simvastatin inhibited the phosphorylation of Jak2, STAT3 (Tyr705) and STAT3 (Ser727) in renal cancer cells in a dose- and time-dependent manner. Quantitative analysis of the p-JAK2/JAK2, p-STAT3 (Tyr705)/STAT3 and p-STAT3 (Ser727)/STAT3 ratio was displayed in each lower panel.</p

Simvastatin inhibited the growth and induced cell apoptosis in RCC tumor xenografts.

<p>Images of the excised tumors (A) and the nude mice (C) were taken from the control and treatment group. The arrows point to the xenografts. (B) Graphs representing the average tumor volumes of A498 xenografts treated with or without simvastatin. (D) Body weight curve of nude mice bearing A498 tumors treated with simvastatin. (E) Representative TUNEL staining (red fluoresence) of A498 renal cancer xenografts. (F) Bar graph showing quantification of the TUNEL positive A498 cells in tumor xenogafts. Data are presented as mean ± SD, *p<0.05.</p

Simvastatin suppressed proliferation and metastasis of A498 cells that were transfected with STAT3 siRNA.

<p>(A) A498 cells were transfected with STAT3 siRNA and treated with simvastatin (8 µM), and the levels of STAT3 was analyzed by western blotting with GAPDH as a control. (B, C) After transfected with STAT3 siRNA, A498 cells were incubated in the absence or presence of simvastatin (8 µM) for 48 h. The cell viability was measured by MTT assay, (B), and cell migration and invasion was measured by transwell assay (C). * p<0.05 or ** p<0.01, compared with the untreated cells (Mock). # p<0.05 or ## p<0.01, compared with the cells transfected with STAT3 siRNA.</p