A549 (A, C) and BEAS-SB (B, D) cells transfected with pLXSNE6E7 constructs and exposed to 0.1 and 10

<p> <b>µg/mL CSC show an increased viability and proliferation rate (mean</b> ± <b>SD, * p<0.05; ** p<0.01, *** p<0.001, unpaired two-tailed Student's t-test).</b></p

HPV16 E6 and E7 oncoproteins have functional activity in lung cells.

<p><b>A</b>: Western blot for p53 protein in A549 and BEAS-2B transfected cells with the corresponding construct. <b>B</b>: Transcriptase-reverse PCR for p53 transcripts in A549 and BEAS-2B transfected cells.</p

Primers used in this study.

<p>Primers used in this study.</p

HPV-16 E6 and E7 expression in A549 (A) and BEAS-2B (B) cells exposed to CSC promotes an increased anchorage-independent growth.

<p>Below: Number of colonies per plate in A549 (left) and BEAS-2B (right) (mean ± SD, unpaired two-tailed Student's t-test. Each plate is representative of three independent experiments).</p

Telomerase (hTERT) and p16^INK4a transcripts are overexpressed in A549 and BEAS-2B cells transfected with pLXSNE6E7 constructs.

<p><b>A</b>: hTERT mRNA detection is significantly increased in BEAS-2B cells transfected with HPV16 E6E7 constructs as detected using qRT-PCR (p=0.0052). <b>B</b>: p16^INK4a detection is significantly increased in BEAS-2B cells as detected using qRT-PCR (mean ± SD, p<0.0001, unpaired two-tailed Student's t-test).</p

Viability of BEAS-2B (A) and A549 (B) lung cells transfected with pLXSN constructs and exposed to cigarette smoke condensate (range: 0–100 μg/mL) (mean ± SD, unpaired two-tailed Student's t-test).

<p>NS: Non-significant.</p

A549 and BEAS-2B lung cells transfected with pLXSNE6E7 constructs show an increased proliferation rate using MTS assays (mean

<p>± <b>SE). </b><b>A</b>, <b>B</b>: Viability of A549 (<b>A</b>) and BEAS-2B (<b>B</b>) lung cells transfected with the empty vector and pLXSNE6E7 constructs. <b>C</b>, <b>D</b>: Proliferation curves of A549 (<b>C</b>) and BEAS-2B (<b>D</b>) cells transfected with PLXSNE6E7 and empty vectors (mean ± SD, * p<0.05; ** p<0.01, *** p<0.001, unpaired two-tailed Student's t-test).</p

CFSE-labeled A549 cells transfected with empty vector and pLXSNE6E7 were exposed during 48

<p> <b>h to 0.1 </b><b>µg/mL of CSC.</b> A representative histogram is shown (mean ± SD, unpaired two-tailed Student's t-test).</p

Supplemental Table 1 from Natal foraging philopatry in eastern Pacific hawksbill turtles

Results (mean % ± 95% confidence intervals in parentheses) from the Bayesian mixed stock analysis from hawksbill rookeries ranked by location to the four regional foraging grounds (RFGs). The two rookeries in RFG2 were combined into a single regional estimate for the regional analysis due to their proximity and genetic similarity

Supplemental Figure 1. from Natal foraging philopatry in eastern Pacific hawksbill turtles

Results of the principal components analysis of the eight foraging grounds with sample sizes ranging from n = 20-117 showing the four clusters that determined the four regional foraging grounds (RFGs). Samples from neighboring foraging grounds with sample sizes ranging from n = 1-7 were pooled into each RFG for the Bayesian mixed stock analysis