1 research outputs found
Synthesis and Characterization of Lipid Immuno-Nanocapsules for Directed Drug Delivery: Selective Antitumor Activity against HER2 Positive Breast-Cancer Cells
Lipid nanocapsules (LNC) are usually
developed as nanocarriers
for lipophilic drug delivery. The surface characteristics of these
colloidal particles are determinant for a controlled and directed
delivery to target tissues with specific markers. We report the development
of immuno-nanocapsules, in which some antibody molecules with different
immuno-specificity are conjugated to the nanocapsule surface, offering
the standardization of a simple method to obtain vectorized nanosystems
with specific recognition properties. Nanocapsules were prepared by
a solvent-displacement technique, producing an oily core coated by
a functional shell of different biocompatible molecules and surface
carboxylic groups. Three different antibodies (one a specific HER2
oncoprotein antibody) were conjugated with these nanoparticles by
the carbodiimide method, which allows the covalent immobilization
of protein molecules through carboxylic surface groups. The immuno-nanocapsules
were completely characterized physico-chemically via electrokinetic
and colloidal stability experiments, confirming the correct immobilization
of these antibody molecules on the colloidal nanoparticles. Also,
additional immunological analyses verified that these IgG-LNC complexes
showed the expected specific immuno-response. Finally, different healthy
and tumoral breast-cell lines were cultured in vitro with Nile-Red-loaded
and docetaxel-loaded HER2 immuno-nanocapsules. The results indicate
that our immuno-nanocapsules can increase their uptake in HER2 overexpressing
tumoral cell lines