Quantitative RT-PCR validation for miR-15a, miR-29a, miR-155 and miR-223 in independent CLL patients.

<p>Relative expression of miR-15a, miR-29a, miR-155 and miR-223 [represented as arbitrary units (a.u.)] was evaluated by individual TaqMan miRNA assays performed in duplicate and normalized to RNU43 (2^−dCt). Box plots indicate the median value (horizontal line) and the 25^th–75^th percentile range (box) while whiskers showing the maximum and minimum values. Values outside this range are shown as outliers (open circles). <i>P</i>-values were determined by the Mann-Whitney U test. In every case, miRNAs downregulated in 13q-H CLL patients relative to 13q-L patients were also found to be downregulated by quantitative RT-PCR. Similar observations were made for miR-155, which was upregulated in 13q-H patients. All comparisons were statistically significant (<i>P</i><0.05).</p

Univariate and multivariate analysis of overall survival in adults with ALL.

<p>Univariate and multivariate analysis of overall survival in adults with ALL.</p

miRNAs significantly deregulated between 13q- CLL subgroups (patients with 80% or more of cells with 13q deletion and patients with less than 80% 13q cells).

<p>Upregulation or downregulation refers to 13q-H relative to13q-L CLL patients.</p><p>miRNA: microRNA.</p>*<p>deregulation shared with 17p/11q CLL patients.</p

Characteristics of patients with ALL included in the study.

<p>Characteristics of patients with ALL included in the study.</p

CLL patients with a high number of 13q- cells can be differenciated based on their expression profile.

<p>Principal component analysis (PCA) plot of CD19+cells from healthy controls (green), CLL with normal FISH (sky blue), 13q-H CLL (red) and 13q-L CLL (dark blue) was carried out using the 28,806 remaining genes after filtering the normalized gene expression matrices to remove the least variable genes (25%). Each sphere represents a single GEP. The result of the PCA shows a cumulative variance of 48.3%, 60.9% and 68.3% corresponding to one, two or three of the initial components, respectively. The expression pattern of CD19+cells from CLL patients is notably different from the gene expression profile of CD19+cells from healthy donors. Of note, the PCA analysis shows that 13q-H CLL patients have a distinctive gene expression profile. By contrast, the gene expression of B lymphocytes from 13q-L CLL and nCLL was similar.</p

Kaplan—Meier plots demonstrating the effect of copy number changes on overall survival (OS) in adult patients with ALL.

<p>(A) OS of the whole cohort of adults with ALL with respect to the presence of deletions in 17p. (B) OS of adults without poor-risk with respect to the presence of deletions in 7p12.2. (C) OS of adults with ALL classified in the poor-risk cytogenetic group with respect to the presence of deletions in 3q26.32. NR, not reached.</p