43 research outputs found
Ordering Transition in Salt-Doped Diblock Copolymers
Lithium salt-doped
block copolymers offer promise for applications
as solid electrolytes in lithium ion batteries. Control of the conductivity
and mechanical properties of these materials for membrane applications
relies critically on the ability to predict and manipulate their microphase
separation temperature. Past attempts to predict the so-called “order–disorder
transition temperature” of copolymer electrolytes have relied
on approximate treatments of electrostatic interactions. In this work,
we introduce a coarse-grained simulation model that treats Coulomb
interactions explicitly, and we use it to investigate the ordering
transition of charged block copolymers. The order–disorder
transition temperature is determined from the ordering free energy,
which we calculate with a high level of precision using a density-of-states
approach. Our calculations allow us to discern a delicate competition
between two physical effects: ion association, which raises the transition
temperature, and solvent dilution, which lowers the transition temperature.
In the intermediate salt concentration regime, our results predict
that the order–disorder transition temperature increases with
salt content, in agreement with available experimental data
Coarse-Grained Ions for Nucleic Acid Modeling
We
present a general coarse-grained model of sodium, magnesium,
spermidine, and chlorine in implicit solvent. The effective potentials
between ions are systematically parametrized using a relative entropy
coarse-graining approach [Carmichael, S. P. and M. S. Shell, J. <i>Phys. Chem. B</i>, <i>116</i>, 8383–93 (2012)]
that maximizes the information retained in a coarse-grained model.
We describe the local distribution of ions in the vicinity of a recently
published coarse-grained DNA model and demonstrate a dependence of
persistence length on ionic strength that differs from that predicted
by Odijk–Skolnick–Fixman theory. Consistent with experimental
observations, we show that spermidine induces DNA condensation whereas
magnesium and sodium do not. This model can be used alongside any
coarse-grained DNA model that has explicit charges and an accurate
reproduction of the excluded volume of dsDNA
Dynamics and Deformation Response of Rod-Containing Nanocomposites
Theoretical and computational studies of polymer nanocomposites have largely focused on spherical inclusions in a polymer matrix. In order to address the influence of particle shape on nanocomposite behavior, extensive Monte Carlo and molecular dynamics simulations are used to examine the structure and deformation behavior of a model polymer upon addition of rods of varying aspect ratios. It is found that, at constant temperature, nanorod length does not meaningfully affect the elastic properties of composites but does affect postyield properties, such as the strain hardening modulus. In contrast, at constant <i>T</i>/<i>T</i><sub>g</sub>, several trends with additive length arise. Examination of the polymer bond autocorrelation function during deformation reveals that longer, dispersed rods induce a broadening of the relaxation spectrum. Nanocomposites show longer bond orientation relaxation times than the pure polymer during all stages of deformation. For the truly nanoscale additives used in this study, polymer mobility is found to be only a weak function of distance to the nearest nanorod so long as the additives did not aggregate
Efficient Free Energy Calculation of Biomolecules from Diffusion-Biased Molecular Dynamics
Recently proposed metadynamics techniques offer an effective
means
for improving sampling in simulations of complex systems, including
polymers and biological macromolecules. One of the drawbacks of such
methods has been the absence of well-defined or effective convergence
criteria. A solution to this problem is considered here in which an
optimal ensemble is introduced to minimize the travel time across
the entire order parameter range of interest. The usefulness of the
proposed approach is illustrated in the context of two systems consisting
of biological molecules dissolved in water. The results presented
in this work indicate that the proposed method is considerably faster
than other existing algorithms for the study of these systems, and
that the corresponding free energy that emerges from the simulations
converges to the exact result
Topological Effects in Isolated Poly[<i>n</i>]catenanes: Molecular Dynamics Simulations and Rouse Mode Analysis
Poly[<i>n</i>]catenanes
are mechanically interlocked
polymers consisting of interlocking ring molecules. Over the years,
researchers have speculated that the permanent topological interactions
within the poly[<i>n</i>]catenane backbone could lead to
unique dynamical behaviors. To investigate these unusual polymers,
molecular dynamics simulations of isolated poly[<i>n</i>]catenanes have been conducted, along with a Rouse mode analysis.
Owing to the mechanical bonds within the molecule, the dynamics of
poly[<i>n</i>]catenanes at short length scales are significantly
slowed and the distribution of relaxation times is broadened; these
same behaviors have been observed in melts of linear polymers and
are associated with entanglement. Despite these entanglement-like
effects, at large length scales poly[<i>n</i>]catenanes
do not relax much slower than isolated linear polymers and are less
strongly impacted by increased segmental stiffness
Mechanical Response of DNA–Nanoparticle Crystals to Controlled Deformation
The
self-assembly of DNA-conjugated nanoparticles represents a
promising avenue toward the design of engineered hierarchical materials.
By using DNA to encode nanoscale interactions, macroscale crystals
can be formed with mechanical properties that can, at least in principle,
be tuned. Here we present <i>in silico</i> evidence that
the mechanical response of these assemblies can indeed be controlled,
and that subtle modifications of the linking DNA sequences can change
the Young’s modulus from 97 kPa to 2.1 MPa. We rely on a detailed
molecular model to quantify the energetics of DNA–nanoparticle
assembly and demonstrate that the mechanical response is governed
by entropic, rather than enthalpic, contributions and that the response
of the entire network can be estimated from the elastic properties
of an individual nanoparticle. The results here provide a first step
toward the mechanical characterization of DNA–nanoparticle
assemblies, and suggest the possibility of mechanical metamaterials
constructed using DNA
Secondary Structure of Rat and Human Amylin across Force Fields
<div><p>The aggregation of human amylin has been strongly implicated in the progression of Type II diabetes. This 37-residue peptide forms a variety of secondary structures, including random coils, α-helices, and β-hairpins. The balance between these structures depends on the chemical environment, making amylin an ideal candidate to examine inherent biases in force fields. Rat amylin differs from human amylin by only 6 residues; however, it does not form fibrils. Therefore it provides a useful complement to human amylin in studies of the key events along the aggregation pathway. In this work, the free energy of rat and human amylin was determined as a function of α-helix and β-hairpin content for the Gromos96 53a6, OPLS-AA/L, CHARMM22/CMAP, CHARMM22*, Amberff99sb*-ILDN, and Amberff03w force fields using advanced sampling techniques, specifically bias exchange metadynamics. This work represents a first systematic attempt to evaluate the conformations and the corresponding free energy of a large, clinically relevant disordered peptide in solution across force fields. The NMR chemical shifts of rIAPP were calculated for each of the force fields using their respective free energy maps, allowing us to quantitatively assess their predictions. We show that the predicted distribution of secondary structures is sensitive to the choice of force-field: Gromos53a6 is biased towards β-hairpins, while CHARMM22/CMAP predicts structures that are overly α-helical. OPLS-AA/L favors disordered structures. Amberff99sb*-ILDN, AmberFF03w and CHARMM22* provide the balance between secondary structures that is most consistent with available experimental data. In contrast to previous reports, our findings suggest that the equilibrium conformations of human and rat amylin are remarkably similar, but that subtle differences arise in transient alpha-helical and beta-strand containing structures that the human peptide can more readily adopt. We hypothesize that these transient states enable dynamic pathways that facilitate the formation of aggregates and, eventually, amyloid fibrils.</p></div
Free Energy of Defects in Ordered Assemblies of Block Copolymer Domains
We investigate commonly occurring defects in block copolymer
thin
films assembled on chemically nanopatterned substrates and predict
their probability of occurrence by computing their free energies.
A theoretically informed 3D coarse grain model is used to describe
the system. These defects become increasingly unstable as the strength
of interactions between the copolymer and the patterned substrate
increases and when partial defects occur close to the top surface
of the film. The results presented here reveal an extraordinarily
large thermodynamic driving force for the elimination of defects.
When the characteristics of the substrate are commensurate with the
morphology of the block copolymer, the probability of creating a defect
is extremely small and well below the specifications of the semiconductor
industry for fabrication of features having characteristic dimensions
on the scale of tens of nanometers. We also investigate how the occurrence
of defect changes when imperfections arise in the underlying patterns
and find that, while defects continue to be remarkably unstable, stretched
patterns are more permissive than compressed patterns
Liquid Crystal-Based Emulsions for Synthesis of Spherical and Non-Spherical Particles with Chemical Patches
We
report the use of liquid crystal (LC)-in-water emulsions for
the synthesis of either spherical or non-spherical particles with
chemically distinct domains located at the poles of the particles.
The approach involves the localization of solid colloids at topological
defects that form predictably at surfaces of water-dispersed LC droplets.
By polymerizing the LC droplets displaying the colloids at their surface
defects, we demonstrate formation of both spherical and, upon extraction
of the mesogen, anisotropic composite particles with colloids located
at either one or both of the poles. Because the colloids protrude
from the surfaces of the particles, they also define organized, chemical
patches with functionality controlled by the colloid surface
Helmholtz free energy in kT versus β<sub>RMSD</sub> for rat and human amylin.
<p>The β<sub>RMSD</sub> is correlated with the number of residues in a β-hairpin. The results for rIAPP are shown using solid lines, while the results for hIAPP are given in dashed lines. The Helmholtz free energy is shown for Amberff99sb*-ILDN with TIP3P (black), Amberff03w with TIP4P2005 (red), and CHARMM22* with TIP4P (blue).</p