Frequency of single vs multiple (2, 3 or 4) vertebrate blood types identified by PCR in T. infestans from Chuquisaca, Bolivia.

*<p>Probability that vectors that fed on the specified host fed on more than one host (one-tailed test).</p>**<p>Total number is less than the column sum because of multiple infections.</p

Variation among habitats in T. cruzi prevalence in T. infestans from Chuquisaca.

*<p>T. cruzi infection prevalence not significantly different among habitats (Likelihood ratio Chi square = 3.56, P>0.05).</p

Percent of vectors from each habitat that tested positive by PCR for 11 different types of vertebrate DNA.

*<p>Probability a blood source is randomly distributed between domestic and peri-domestic habitat types.</p

Vector movement between domestic and peri-domestic structures.

1<p>Number of vectors positive for human DNA collected from peri-domestic habitats.</p>2<p>Number of vectors positive for cow, chicken, pig or goat DNA collected in houses.</p>3<p>Likelihood Chi-square = 32.86, p<0.0001).</p

Pairwise probabilities of occurrence of two types of DNA in the same T. infestans specimen from Chuquisaca.

*<p>Likelihood ratio Chi square (P-values) for pair co-occurring less than (⇓) or greater than (⇑) expected by chance.</p

Primer sequence and PCR product size for species-specific assays.

1<p>Nuclear Alu Yb6 subfamily <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0003585#pone.0003585-WisniveskyColli1" target="_blank">[25]</a>.</p>2<p>Nuclear SINE (Short Interspersed Nuclear Element) <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0003585#pone.0003585-Minter1" target="_blank">[22]</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0003585#pone.0003585-Pineda1" target="_blank">[23]</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0003585#pone.0003585-Zeledon1" target="_blank">[26]</a>.</p>3<p>Mitochondrial subunit 8 ATP synthase gene <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0003585#pone.0003585-Sasaki1" target="_blank">[24]</a>.</p>4<p>Nuclear 195 bp repetitive DNA <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0003585#pone.0003585-AbadFranch1" target="_blank">[30]</a>.</p

For domestic and peri-domestic habitats, we show the percentage of each type of blood meal found in that habitat and the <i>T. cruzi</i> prevalence in insect vectors that had fed on each blood source.

<p>Vectors from domestic vs. peri-domestic habitats vary in their blood meal source. Almost all the insects that had fed on humans were collected from houses. Detection of cow, chicken pig or goat DNA in vectors collected from houses indicates a migrant from a peri-domestic to domestic habitat. Detection of human DNA in a vector collected from a peri-domestic habitat indicates migration from domestic to peri-domestic habitat.</p

The 188-bp PCR product amplified from DNA in 2% agarose gel stained with ethidium-bromide

<p><b>Copyright information:</b></p><p>Taken from "PCR reveals significantly higher rates of infection than microscopy in the Chagas vector, : High rates found in Chuquisaca, Bolivia"</p><p>http://www.biomedcentral.com/1471-2334/7/66</p><p>BMC Infectious Diseases 2007;7():66-66.</p><p>Published online 27 Jun 2007</p><p>PMCID:PMC1920523.</p><p></p> Lane 1, 100 bp ladder; lane 2, positive control; lane 3, negative control, water as template; lanes 6, 10 and 12, negative results; lanes 4, 5, 7, 8, 9 and 11, positive results

Polar contacts in the model between 17.2 and β1-AR.

<p>MC: Main Chain; SC: Side Chain.</p

Cp (crossing point) vs. serial dilutions for chicken (black circle), <i>Canis sp.</i> (blue square) and human (red triangle) assays in triplicate.

<p>Cp (crossing point) vs. serial dilutions for chicken (black circle), <i>Canis sp.</i> (blue square) and human (red triangle) assays in triplicate.</p