8 research outputs found

    Additional file 1: of A new high-throughput screening-compatible gap junctional intercellular communication assay

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    Expression of connexin43 in LN215 and HOS cells. To determine whether LN215 and HOS cells express a major connexin, connexin43, we performed immunoblot analysis with anti-connexin43 antibody (C13720, Transduction Laboratories). HeLa, LN215 and HOS cells were lysed with PBS containing 1 % Triton X-100 and cOmplete™ protease inhibitor cocktail (Roche) before centrifugation at 15,000 × g for 10 min at 4 °C. After the BCA protein assay, 20 μg of protein was used for immunoblotting. Since HeLa cells do not express connexin43 [24], they were used as a negative control. Connexin43 (arrow head) was detected in LN215 and HOS lysates. (TIFF 271 kb
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