Additional file 3: Figure S3. of Negative effect of cyclin D1 overexpression on recurrence-free survival in stage II-IIIA lung adenocarcinoma and its expression modulation by vorinostat in vitro

The effect of vorinostat on expression of cyclins. The expression levels of cyclin E, cyclin A2, and cyclin B1 were analyzed in A549, H460, and H226 cells treated with vorinostat and/or B[a]P. Western blotting was performed according to standard procedures. Antibodies against cyclin E (HE12, #4129), cyclin A2 (BF683, #4656), and cyclin B1 (#4138) were purchased from Cell Signaling Technology. (TIFF 1815 kb

Additional file 1: Figure S1. of Negative effect of cyclin D1 overexpression on recurrence-free survival in stage II-IIIA lung adenocarcinoma and its expression modulation by vorinostat in vitro

Recurrence-free survival in stage II-IIIA NSCLC. Recurrence-free survival was compared according to expression statuses of cyclin A2, cyclin E, and p16. Data were stratified according to histology. The yellow and blue lines indicate groups with and without abnormal expression of each protein, respectively. P–values were based on the log-rank test. (TIFF 2996 kb

Additional file 2: Figure S2. of Negative effect of cyclin D1 overexpression on recurrence-free survival in stage II-IIIA lung adenocarcinoma and its expression modulation by vorinostat in vitro

Effect of low dose vorinostat on cell proliferation. A549 cells were pre-treated with 5 μM B[a]P for 9 days and then incubated in combination with 1 μM vorinostat for the indicated hours. The total number of viable cells at each time point was determined by MTT assay. The y-axis indicates cell numbers relative to time zero. Each value represents mean ± standard deviation of three experiments. (TIFF 1042 kb

Demographic characteristics of the patients (n = 44).

<p>NSCLC, non-small-cell lung cancer; NOS; not otherwise specified.</p

Diagnostic algorithm of study patients.

<p>EBUS-TBNA, endobronchial ultrasound-guided transbronchial needle aspiration; EUS-FNA-B/E, transesophageal bronchoscopic ultrasound-guided fine-needle aspiration.</p

The six cases of increased mediastinal stage after addition of EUS-FNA-B/E.

<p>EBUS-TBNA: endobronchial ultrasound-guided transbronchial needle aspiration; EUS-FNA-B/E: transesophageal bronchoscopic ultrasound-guided fine-needle aspiration.</p

Mediastinal lymph nodes examined by endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA) and transesophageal bronchoscopic ultrasound-guided fine-needle aspiration (EUS-FNA-B/E).

<p>EBUS-TBNA: endobronchial ultrasound-guided transbronchial needle aspiration; EUS-FNA-B/E: transesophageal bronchoscopic ultrasound-guided fine-needle aspiration.</p

Additional file 2: of Bronchial biopsy specimen as a surrogate for DNA methylation analysis in inoperable lung cancer

Methylation levels of seven CpGs across different kinds of samples. Methylation levels of seven CpGs at PCDH7 (A), VIPR2 (B), HBP1 (C), CD34 (D), HOXA9 (E), SOX17 (F), and HOXD13 (G) were compared among six different kinds of samples: bronchial washing samples from 76 lung cancer patients (BW_cancer), 60 healthy individuals (BW_control), bronchial biopsies from 8 lung cancer patients (LC_biopsy), 42 lung tumor (LC_tumor) and matched normal tissues (LC_normal), and sputum from 12 lung cancer patients (LC_sputum). Y-axis indicates β-values from the 450K array. (TIFF 3249 kb

Univariate and multivariate hazard ratios for mortality in a biopsy-proven CPFE cohort.

<p>Definitions of abbreviations: FEV1 = forced expiratory volume in 1 second; FVC = forced vital capacity; DLco = diffusing capacity for carbon monoxide; CPI = composite physiologic index; UIP = usual interstitial pneumonia; FS = fibrosis score; EI = emphysema index.</p><p>*Hazard ratios reported for 10-unit change.</p><p>Univariate and multivariate hazard ratios for mortality in a biopsy-proven CPFE cohort.</p

Baseline subject characteristics of two cohorts with combined pulmonary fibrosis and emphysema.

<p>Definitions of abbreviations: IIP = idiopathic interstitial pneumonia; FEV1 = forced expiratory volume in 1 second; FVC = forced vital capacity; DLco = diffusing capacity for carbon monoxide; UIP = usual interstitial pneumonia; NSIP = nonspecific interstitial pneumonia; RB-ILD = respiratory bronchiolitis-associated interstitial lung disease; DIP = desquamative interstitial pneumonia.</p><p>Baseline subject characteristics of two cohorts with combined pulmonary fibrosis and emphysema.</p