3 research outputs found
Modified Rheokinetic Technique to Enhance the Understanding of Microcapsule-Based Self-Healing Polymers
A modified rheokinetic technique was developed to monitor
the polymerization
of healing monomers in a microcapsule-based, self-healing mimicking
environment. Using this modified technique, monomers active toward
ring-opening metathesis polymerization (ROMP) were either identified
or disregarded as candidates for incorporation in self-healing polymers.
The effect of initiator loading on the quality and speed of healing
was also studied. It was observed that self-healing polymers have
upper and lower temperature limits between which the healing mechanism
performs at optimal levels. Also, a study of the quality of healing
cracks of different thicknesses was performed, and it was discovered
that above a critical crack thickness value, the quality of self-healing
diminishes substantially; reasons for this phenomenon are discussed
in detail
Volatile atmospheric pressure chemical ionisation mass spectrometry headspace analysis of E. coli and S. aureus
Identifying the characteristics of bacterial species can improve treatment outcomes and mass spectrometry methods have been shown to
be capable of identifying biomarkers of bacterial species. This study is
the first to use volatile atmospheric pressure chemical ionisation mass
spectrometry to directly and non-invasively analyse the headspace of
E. coli and S. aureus bacterial cultures, enabling major biological
classification at species level (Gram negative/positive respectively).
Four different protocols were used to collect data, three utilising
discrete 5 min samples taken between 2 and 96 h after inoculation and
one method employing 24 h continuous sampling. Characteristic
marker ions were found for both E. coli and S. aureus. A model to
distinguish between sample types was able to correctly identify the
bacteria samples after sufficient growth (24–48 h), with similar results
obtained across different sampling methods. This demonstrates that
this is a robust method to analyse and classify bacterial cultures
accurately and within a relevant time frame, offering a promising
technique for both clinical and research application
Supplementary information files for Volatile atmospheric pressure chemical ionisation mass spectrometry headspace analysis of E. coli and S. aureus
© the authors, CC-BY NC 4.0Supplementary files for article Volatile atmospheric pressure chemical ionisation mass spectrometry headspace analysis of E. coli and S. aureusIdentifying the characteristics of bacterial species can improve treatment outcomes and mass spectrometry methods have been shown to be capable of identifying biomarkers of bacterial species. This study is the first to use volatile atmospheric pressure chemical ionisation mass spectrometry to directly and non-invasively analyse the headspace of E. coli and S. aureus bacterial cultures, enabling major biological classification at species level (Gram negative/positive respectively). Four different protocols were used to collect data, three utilising discrete 5 min samples taken between 2 and 96 h after inoculation and one method employing 24 h continuous sampling. Characteristic marker ions were found for both E. coli and S. aureus. A model to distinguish between sample types was able to correctly identify the bacteria samples after sufficient growth (24–48 h), with similar results obtained across different sampling methods. This demonstrates that this is a robust method to analyse and classify bacterial cultures accurately and within a relevant time frame, offering a promising technique for both clinical and research applications</p