Families of sodium and calcium ion channel subtypes identified in <i>H</i>. <i>verbana</i> CNS transcriptome.

<p>Trees were produced in the same manner as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0201206#pone.0201206.g004" target="_blank">Fig 4</a>. The putative <i>H</i>. <i>verbana</i> sodium and calcium ion channel subtypes are listed in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0201206#pone.0201206.t004" target="_blank">Table 4</a> with accession numbers and putative associated membrane currents.</p

Gene ontology distribution of the annotated <i>H</i>. <i>verbana</i> CNS transcriptome.

<p>The absolute values of the top 10 most abundant sequence annotations for each classification are represented at a GO level of 3. Major GO categorizations are classified into biological process, molecular function, and cellular component.</p

Biogenic amine and GABA receptor accession numbers from CNS transcriptome.

<p>Biogenic amine and GABA receptor accession numbers from CNS transcriptome.</p

RNA-seq kallisto counts for ion channels and housekeeping genes identified in the <i>H</i>. <i>verbana</i> CNS transcriptome.

<p>Horizontal bar plot ordered based on read alignment with color coordinating to channel families. Read counts were normalized using TPM method. Inset displays zoomed in visualization of channels where TPM fell below 1000. Housekeeping genes are displayed with a scale bar that is an order of magnitude greater than that of the ion channels.</p

Comparison of previously described innexin sequence identity and expression against CNS transcriptome.

<p>Comparison of previously described innexin sequence identity and expression against CNS transcriptome.</p

Blast hit species distribution of <i>H</i>. <i>verbana</i> nervous system transcriptome.

<p>(A). Frequency of species assigned to each contig from total BLAST hits (up to 20 hits per contig) against NCBI’s validated RefSeq database of Animalia protein sequences with an e-value threshold = 10⁻⁵. (B). Frequency of species assigned to each contig from the highest scoring BLAST hit for each contig.</p

Neurotransmitter-associated enzymes and transporters identified in the <i>H</i>. <i>verbana</i> CNS transcriptome.

<p>Trees were produced in the same manner as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0201206#pone.0201206.g004" target="_blank">Fig 4</a>. Transmitter-related subtypes and accession numbers can be found in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0201206#pone.0201206.t008" target="_blank">Table 8</a>.</p

KCNQ and KCNK channel families identified in <i>H</i>. <i>verbana</i> CNS transcriptome.

<p>Trees were produced in the same manner as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0201206#pone.0201206.g004" target="_blank">Fig 4</a>. <i>H</i>. <i>verbana</i> KCNQ and KCNK channel subtypes, putative membrane currents, and accession numbers can be found in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0201206#pone.0201206.t003" target="_blank">Table 3</a>.</p

Glutamate and acetylcholine receptor accession numbers from CNS transcriptome.

<p>Glutamate and acetylcholine receptor accession numbers from CNS transcriptome.</p

Ionotropic (kainate- and NMDA-like) and metabotropic glutamate receptors identified in the <i>H</i>. <i>verbana</i> CNS transcriptome.

<p>Trees were produced in the same manner as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0201206#pone.0201206.g004" target="_blank">Fig 4</a>. <i>H</i>. <i>verbana</i> glutamate receptor subtypes and accession numbers can be found in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0201206#pone.0201206.t007" target="_blank">Table 7</a>.</p