Schematic showing the alignment of Sanger sequences to scaffolds in benthgenome.com and solgenomics.net.

<p>Schematic showing the alignment of Sanger sequences to scaffolds in benthgenome.com and solgenomics.net.</p

Schematic of pBIN19-mGFP-ER and Tn5393 and their integration site in 16c.

<p><b>(A–B)</b> Schematic of genes within the T-DNA and transposon integrated in 16c genome. The sequence junctions between <i>N</i>. <i>benthamiana</i> genome-T-DNA-transposon-<i>N</i>. <i>benthamiana</i> genome are highlighted, GenBank accession number KY464890. <b>(C)</b> Log scale density plot of sequence reads aligning to T-DNA, transposon and flanking <i>N</i>. <i>benthamiana</i> genomic regions. <b>(D)</b> Location of primers used for amplification of various regions to confirm site of integration. <b>(E)</b> Log scale density plot of RNAseq reads aligning to T-DNA and transposon. <b>(F)</b> Gel image showing the endpoint (35 cycles) PCR amplicons of the T-DNA insert in 16c with LAB <i>N</i>. <i>benthamiana</i> used as a control. Expected sizes with primer pair B+E is 4174 nt and D+F is 4167 nt. NTC, no template control, 1 kb DNA Ladder (GeneRuler^™). <b>(G)</b> Leaves of <i>N</i>. <i>benthamiana</i> 16c photographed under UV light, showing mobile silencing, 14 days after local induction of RNAi at a lower leaf. Abbreviations are: RB: Right Border, pNOS: nopaline synthase promoter, <i>NPTII</i>: neomycin phosphotransferase II encoding gene, tNOS: nopaline synthase terminator, p35S: Cauliflower mosaic virus 35S promoter. IR: inverted terminal repeat, <i>tnpA</i> transposase gene, res: recombination region, <i>tnpR</i> resolvase gene, IS1133: an insertion element, <i>strA</i> and <i>strB</i>: streptomycin resistance genes, Nb Genome: Flanking DNA of <i>N</i>. <i>benthamiana</i>.</p

List of primers used in this study.

<p>List of primers used in this study.</p