Effect of DHC on Heterochromatin Silencing in S. cerevisiae at the HMR or HML Loci

<div><p>(A) DMSO negative control, 1 M nicotinamide positive control, and 50 mM DHC.</p><p>(B and C) DHC and splitomicin, respectively, cause dose-dependent increases in heterochromatin silencing and colony formation.</p></div

DHC Inhibits Human NAD⁺-Dependent Deacetylases In Vitro

<div><p>(A) Dose-dependent inhibition of SIRT1 (<i>y</i> = −19.98x + 96.431).</p><p>(B) Dose-dependent inhibition of SIRT2 (<i>y</i> = −20.79x + 101.34). Averages ± standard error from two separate experiments are shown.</p></div

DHC Increases p53 Acetylation, Cytotoxicity, and Apoptosis in the TK6 Cell Line

<div><p>(A) DHC increases p53 lysine 373 and 382 acetylation in a dose-dependent manner in the TK6 cell line following a 24-h exposure period. The immunoblot is representative of three separate experiments.</p><p>(B) DHC increases cytotoxicity in a dose-dependent manner following a 24-h exposure to DHC. The average ± standard error for each of three experiments is shown.</p><p>(C) A 5-mM dose of DHC increases apoptosis at the 6-h time point in the TK6 cell line. A combination of annexin V fluorescein isothiocyanate and propidium iodide staining was used to discriminate among apoptotic (lower-right box) and necrotic (upper-right box) cells. Figures are representative of three separate experiments.</p><p>(D) DHC increases apoptosis in a dose-dependent manner in the TK6 cell line at the 6-h time point. The average ± standard error for three experiments is shown.</p></div

DHC Reverses the Lethal Phenotype of Galactose-Induced Plasmid <i>SIR2</i> Overexpression

<p>Two-fold yeast serial dilutions were used for this assay. Growth is observed on 0.1% DMSO and 750 μM DHC in dextrose medium. The lethal <i>SIR2</i> phenotype is observed on the 0.1% DMSO galactose medium but is partially reversed on the 750 μM DHC galactose medium.</p

Additional file 1 of Analytical performance of Envisia: a genomic classifier for usual interstitial pneumonia

RNA-seq raw counts of samples used in Envisia analytical verification studies. This file includes the raw expression counts of 190 classifier genes across all samples (n=292) used in the analytical verification studies described in this manuscript; each row represents a gene and each column represents a sample. (CSV 199 kb