Social equity impacts of increased water for irrigation

Water distributionIrrigation waterEquityTanksCanal irrigationVillagesEmploymentWagesGenderSocial aspectsWater users associationsFarmers

Secrets of a Successful Pathogen: Legionella Resistance to Progression Along the Autophagic Pathway

To proliferate within phagocytes, Legionella pneumophila relies on Type IV secretion to modulate host cellular pathways. Autophagy is an evolutionarily conserved degradative pathway that captures and transfers a variety of microbes to lysosomes. Biogenesis of L. pneumophila-containing vacuoles and autophagosomes share several features, including endoplasmic reticulum (ER)-derived membranes, contributions by the host GTPases Rab1, Arf1 and Sar1, and a final destiny in lysosomes. We discuss morphological, molecular genetic, and immunological data that support the model that, although A/J mouse macrophages efficiently engulf L. pneumophila within autophagosomal membranes, the Type IV effector proteins DrrA/SidM, LidA, and RalF prolong association with the ER. By inhibiting immediately delivery to lysosomes, the bacteria persist in immature autophagosomal vacuoles for a period sufficient to differentiate into an acid-resistant, replicative form. Subsequent secretion of the Type IV effector LepB releases the block to autophagosome maturation, and the adapted progeny continue to replicate within autophagolysosomes. Accordingly, L. pneumophila can be exploited as a genetic tool to analyze the recruitment and function of the macrophage autophagy pathway

Interleukin-33 contributes to both M1 and M2 chemokine marker expression in human macrophages

Abstract Background Interleukin-33 is a member of the IL-1 cytokine family whose functions are mediated and modulated by the ST2 receptor. IL-33-ST2 expression and interactions have been explored in mouse macrophages but little is known about the effect of IL-33 on human macrophages. The expression of ST2 transcript and protein levels, and IL-33-mediated effects on M1 (i.e. classical activation) and M2 (i.e. alternative activation) chemokine marker expression in human bone marrow-derived macrophages were examined. Results Human macrophages constitutively expressed the membrane-associated (i.e. ST2L) and the soluble (i.e. sST2) ST2 receptors. M2 (IL-4 + IL-13) skewing stimuli markedly increased the expression of ST2L, but neither polarizing cytokine treatment promoted the release of sST2 from these cells. When added to naïve macrophages alone, IL-33 directly enhanced the expression of CCL3. In combination with LPS, IL-33 blocked the expression of the M2 chemokine marker CCL18, but did not alter CCL3 expression in these naive cells. The addition of IL-33 to M1 macrophages markedly increased the expression of CCL18 above that detected in untreated M1 macrophages. Similarly, alternatively activated human macrophages treated with IL-33 exhibited enhanced expression of CCL18 and the M2 marker mannose receptor above that detected in M2 macrophages alone. Conclusions Together, these data suggest that primary responses to IL-33 in bone marrow derived human macrophages favors M1 chemokine generation while its addition to polarized human macrophages promotes or amplifies M2 chemokine expression.http://deepblue.lib.umich.edu/bitstream/2027.42/78250/1/1471-2172-11-52.xmlhttp://deepblue.lib.umich.edu/bitstream/2027.42/78250/2/1471-2172-11-52.pdfPeer Reviewe

A systemic granulomatous response to Schistosoma mansoni eggs alters responsiveness of bone marrow‐derived macrophages to Toll‐like receptor agonists

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/141008/1/jlb0314.pd

Streamlined and Cost-Effective Genomic DNA Extraction Method for Lichens, Mushrooms, and Endolichenic Fungi: Enabling DNA Barcoding and Molecular Research

Extraction of nucleic acids in pure form from organisms is of paramount importance for DNA based identification and other molecular studies. Over the past few decades, DNA-barcoding has emerged as a powerful technique, facilitating species identification across various ‘difficult to identify’ life-forms. Fungi, being an immensely diverse group of microorganisms, contribute significantly to global biodiversity, with estimates ranging from 2.2 to 3.8 million species. However, a vast majority of this diversity remains unidentified, and many fungal species are considered cryptic. Therefore, numerous large- and small-scale DNA-barcoding projects are being conducted worldwide to unravel this rich biodiversity. However, the rigidity and high complex polysaccharides content of fungal cell-wall presents a significant obstacle, making the extraction of high-quality genomic DNA a challenging task across varied fungal organisms. In this study, we employed a modified CTAB based method to isolate and purify high-quality PCR-amplifiable genomic DNA primarily from lichens and tested it on other fungal life forms as well, including, mushrooms, endolichenic fungi, and parasitic fungi. Remarkably, the isolated DNA proved successful as a template in PCR reactions, serving the purposes of DNA barcoding, RAPD as well as for metagenomic analysis effectively. This versatile protocol demonstrated its utility across all the fungal life forms investigated in this study, offering a universal, cost-effective, and efficient approach for fungal DNA isolation

Association of preeclampsia with anthropometric measures and blood pressure in Indian children.

BACKGROUND AND OBJECTIVE: Birth weight and post-natal growth are important predictors of adult health. Preeclampsia (PE) is associated with low birth weight and may have long term effects on the health of the children. The current study aims to compare anthropometry and blood pressure between children of mothers with and without PE in an Indian cohort. METHODS: We studied children born to women with (PE; n = 211) and without preeclampsia (non-PE; n = 470) at Bharati Hospital, Pune, India. Anthropometry and blood pressure were measured in children at 3-7 years of age. Weight and height Z-scores were calculated using the WHO 2006 growth reference. Independent t-tests were used to compare means between the two groups, and associations between preeclampsia and child outcomes were analyzed using multiple linear regression, adjusting for potential confounders. RESULTS: Weight and height Z-scores (p = 0.04 and 0.008), and subscapular skinfold thickness (p = 0.03) were higher among children of PE compared with children of non-PE mothers. Systolic blood pressure was also higher in children of PE mothers (1.70 mmHg [95% CI 0.05, 2.90] p = 0.006). BMI and diastolic blood pressure did not differ between groups. In regression models adjusted for newborn weight and gestational age, current age and sex, and maternal height, BMI and socio-economic status, children of PE mothers had higher weight Z-score (0.27 SD [95%CI 0.06, 0.48] p = 0.01), height Z-score (0.28 SD [95%CI 0.09, 0.47] p = 0.005), and subscapular skinfold thickness (0.38 mm [95%CI 0.00, 0.76] p = 0.049). A trend for higher systolic blood pressure (1.59 mmHg [95%CI -0.02, 3.20] p = 0.053) in the children was also observed in the adjusted model. The difference in systolic blood pressure was attenuated after adjusting further for the child's weight and height (1.09 mmHg [95%CI -0.48, 2.67] p = 0.17). There was no evidence of differences in effects between boys and girls. CONCLUSION: Children of PE mothers were taller and heavier, and had higher systolic blood pressure, partly explained by their increased body size, than children of non-PE mothers. In utero exposure to preeclampsia may increase the risk of future cardiovascular disease

Serum Amyloid P Therapeutically Attenuates Murine Bleomycin-Induced Pulmonary Fibrosis via Its Effects on Macrophages

Macrophages promote tissue remodeling but few mechanisms exist to modulate their activity during tissue fibrosis. Serum amyloid P (SAP), a member of the pentraxin family of proteins, signals through Fcγ receptors which are known to affect macrophage activation. We determined that IPF/UIP patients have increased protein levels of several alternatively activated pro-fibrotic (M2) macrophage-associated proteins in the lung and monocytes from these patients show skewing towards an M2 macrophage phenotype. SAP therapeutically inhibits established bleomycin-induced pulmonary fibrosis, when administered systemically or locally to the lungs. The reduction in aberrant collagen deposition was associated with a reduction in M2 macrophages in the lung and increased IP10/CXCL10. These data highlight the role of macrophages in fibrotic lung disease, and demonstrate a therapeutic action of SAP on macrophages which may extend to many fibrotic indications caused by over-exuberant pro-fibrotic macrophage responses

Continental extension of northern Gondwana margin in the Eastern Himalaya: Constraints from geochemistry and U–Pb zircon ages of mafic intrusives in the Siang window, Arunachal Himalaya, India

We report new U–Pb zircon age and whole-rock geochemical data from the Pangin mafic intrusive rocks of the Siang window, eastern Himalayas. These mafic rocks are medium to coarse-grained gabbros, consisting mainly of plagioclase and clinopyroxene with accessory phases (hornblende $+$ Fe–Ti oxides) that retain granular and interlocking texture. Geochemically, they display enriched-mid oceanic ridge basalt (E-MORB) affinity characterized by moderate to slightly fractionated REE patterns marked by $(\mathrm{La}/\mathrm{Yb})_{\mathrm{N}} = 2.65-3.99$. Their geochemical characteristics suggest that the parental magmas of these rocks were formed by medium to higher degrees (∼12–28%) of partial melting similar to that of the asthenospheric mantle in the garnet-spinel transition zone. Magmatic zircons from two gabbros yield U–Pb ages of 521.50 $\sim$ 2.53 Ma and 568 ${\pm }$ 2 Ma. This new age reveals two pulses of Late Neoproterozoic and Early Cambrian mafic magmatism that are inconsistent with the temporal distribution of Paleozoic magmatism in the Siang window of the Eastern Himalayas. However, based on the results of this study and the correlation of continental extensional mafic magmatism in the Northwest Himalaya, we suggest that investigated mafic intrusive rocks might have been generated in an extensional tectonic environment during the long-lasting Pan-African orogenic cycle of the late Neoproterozoic to early Cambrian which ended with the formation of the Gondwana supercontinent

Continental extension of northern Gondwana margin in the Eastern Himalaya: Constraints from geochemistry and U–Pb zircon ages of mafic intrusives in the Siang window, Arunachal Himalaya, India

We report new U–Pb zircon age and whole-rock geochemical data from the Pangin mafic intrusive rocks of the Siang window, eastern Himalayas. These mafic rocks are medium to coarse-grained gabbros, consisting mainly of plagioclase and clinopyroxene with accessory phases (hornblende $+$ Fe–Ti oxides) that retain granular and interlocking texture. Geochemically, they display enriched-mid oceanic ridge basalt (E-MORB) affinity characterized by moderate to slightly fractionated REE patterns marked by $(\mathrm{La}/\mathrm{Yb})_{\mathrm{N}} = 2.65-3.99$. Their geochemical characteristics suggest that the parental magmas of these rocks were formed by medium to higher degrees (∼12–28%) of partial melting similar to that of the asthenospheric mantle in the garnet-spinel transition zone. Magmatic zircons from two gabbros yield U–Pb ages of 521.50 $\sim$ 2.53 Ma and 568 ${\pm }$ 2 Ma. This new age reveals two pulses of Late Neoproterozoic and Early Cambrian mafic magmatism that are inconsistent with the temporal distribution of Paleozoic magmatism in the Siang window of the Eastern Himalayas. However, based on the results of this study and the correlation of continental extensional mafic magmatism in the Northwest Himalaya, we suggest that investigated mafic intrusive rocks might have been generated in an extensional tectonic environment during the long-lasting Pan-African orogenic cycle of the late Neoproterozoic to early Cambrian which ended with the formation of the Gondwana supercontinent

Polymer nanoparticles pass the plant interface

As agriculture strives to feed an ever-increasing number of people, it must also adapt to increasing exposure to minute plastic particles. To learn about the accumulation of nanoplastics by plants, we prepared well-defined block copolymer nanoparticles by aqueous dispersion polymerisation. A fluorophore was incorporated via hydrazone formation and uptake into roots and protoplasts of Arabidopsis thaliana was investigated using confocal microscopy. Here we show that uptake is inversely proportional to nanoparticle size. Positively charged particles accumulate around root surfaces and are not taken up by roots or protoplasts, whereas negatively charged nanoparticles accumulate slowly and become prominent over time in the xylem of intact roots. Neutral nanoparticles penetrate rapidly into intact cells at the surfaces of plant roots and into protoplasts, but xylem loading is lower than for negative nanoparticles. These behaviours differ from those of animal cells and our results show that despite the protection of rigid cell walls, plants are accessible to nanoplastics in soil and water