Experimenting with Orange Pavement Markings on Indiana Roadways

This session presents the status of experimental orange pavement markings on Indiana roadways. The presentation includes a summary of the MUTCD experimentation request and an evaluation of orange pavement marking types— including tapes and paints with a focus on color and retroreflectivity retention. The effects of driving behavior on the safety of work zones, vehicle lane positioning, and public opinion are also discussed

Real-time assembly of ribonucleoprotein complexes on nascent RNA transcripts.

Cellular protein-RNA complexes assemble on nascent transcripts, but methods to observe transcription and protein binding in real time and at physiological concentrations are not available. Here, we report a single-molecule approach based on zero-mode waveguides that simultaneously tracks transcription progress and the binding of ribosomal protein S15 to nascent RNA transcripts during early ribosome biogenesis. We observe stable binding of S15 to single RNAs immediately after transcription for the majority of the transcripts at 35 °C but for less than half at 20 °C. The remaining transcripts exhibit either rapid and transient binding or are unable to bind S15, likely due to RNA misfolding. Our work establishes the foundation for studying transcription and its coupled co-transcriptional processes, including RNA folding, ligand binding, and enzymatic activity such as in coupling of transcription to splicing, ribosome assembly or translation

Session 5: Banking, Capital Markets, and The Crypto Revolution - A Look Back and Projection of the Future of FinTech

In Session Five of the SITIE 2023 Symposium: Enabling Innovation in Law and Society, Joseph M. Vincent moderated as the four panelists, Joseph R. Cutler, Lawrence Kaplan, Youssef Sneifer, and Jill Williamson, discussed banking, capital markets, and the crypto revolution by looking back and projecting the future of the financial technology (FinTech) industry. The discussion commenced with a conversation on banking deposits, then moved into a discussion on cryptocurrency companies and the challenges they have faced in recent years in the banking industry. The panelists further discussed artificial intelligence (AI) technology’s impact on FinTech, open banking, and challenges facing cryptocurrency (crypto) going forward

Anomalous Radio-Wave Scattering from Interstellar Plasma Structures

This paper considers scattering screens that have arbitrary spatial variations of scattering strength transverse to the line of sight, including screens that are spatially well confined, such as disks and filaments. We calculate the scattered image of a point source and the observed pulse shape of a scattered impulse. The consequences of screen confinement include: (1) Source image shapes that are determined by the physical extent of the screen rather than by the shapes of much-smaller diffracting microirregularities. These include image elongations and orientations that are frequency dependent. (2) Variation with frequency of angular broadening that is much weaker than the trademark \nu^{-2} scaling law (for a cold, unmagnetized plasma), including frequency-independent cases; and (3) Similar departure of the pulse broadening time from the usually expected \nu^{-4} scaling law. We briefly discuss applications that include scattering of pulses from the Crab pulsar by filaments in the Crab Nebula; image asymmetries from Galactic scattering of the sources Cyg X-3, Sgr A*, and NGC 6334B; and scattering of background active galactic nuclei by intervening galaxies. We also address the consequences for inferences about the shape of the wavenumber spectrum of electron density irregularities, which depend on scaling laws for the image size and the pulse broadening. Future low-frequency (< 100 MHz) array observations will also be strongly affected by the Galactic structure of scattering material. Our formalism is derived in the context of radio scattering by plasma density fluctuations. It is also applicable to optical, UV and X-ray scattering by grains in the interstellar medium.Comment: 21 pages, LaTeX2e with AASTeX-4.0, 6 PostScript figures, accepted by ApJ, revised version has minor changes to respond to referee comments and suggestion

Preparation of Isotopically Labeled Ribonucleotides for Multidimensional NMR Spectroscopy of RNA

A general method for large scale preparation of uniformly isotopically labeled ribonucleotides and RNAs is described. Bacteria are grown on isotopic growth medium, and their nucleic acids are harvested and degraded to mononucleotides. These are enzymatically converted into ribonucleoside triphosphates, which are used in transcription reactions in vitro to prepare RNAs for NMR studies. For 15N-labeling, E.coli is grown on15N-ammonium sulfate, whereas for 13C-labeling, Methylophilus methylotrophus is grown on 13C-methanol, which is more economical than 13C-glucose. To demonstrate the feasibility and utility of this method, uniformly 13C-labeled ribonucleotides were used to synthesize a 31 nucleotide HIV TAR RNA that was analyzed by 3D-NMR. This method should find widespread use in the structural analysis of RNA by NMR

Surveying Consumer Understanding & Sentiment Of VR

Since the resurgence of consumer-grade virtual reality (VR), VR has successfully established itself on the consumer market. As with any emerging technology, differences can exist between how industry / academia view the technology and how consumers perceive it. We present results from a survey (N=210) conducted into consumer perception and attitudes towards VR. We report sentiment towards VR is positive. We show the associations linked with VR by our respondents match the defining characteristics of VR identified by experts in the literature (a fully virtual view, immersion, and head-worn technology). We identify 3 additional concepts associated with VR by our respondents: video games, futurism, and price. However, our results also show consumer expectations for VR are fixated around "VR for gaming" and suggest VR has to an extent been pigeonholed as primarily being a gaming device

Bystander Interruption of VR Users

Research has begun to investigate the interruption of VR users but lacks an understanding of how social factors (setting / relationship to the VR user) might impact the interruption and why bystanders interrupt as they do. We conducted a survey (N=100) into bystander comfort when interrupting a VR user (known / unknown) in 4 settings (private spaces, public spaces, private transport, public transport) and their willingness to use a range of interruption strategies. Our results suggest relationship to the VR user is more influential than setting when considering comfort and acceptability of interruption strategy. A follow-up lab study (N=16) investigated bystander interruption of a known VR user in a private setting. Most used a combination of speech and touch to interrupt though a subset used unconventional, improvised strategies we term playful interruptions. With the recent inclusion of open ear audio in VR headsets and preference towards verbal interruptions in our results we conducted a survey (N=76) into the consumer response to open ear audio in VR headsets. We report open ear audio was used regularly by 52.6% of our respondents 60% of who rate their experience with it positively

Finding Radio Pulsars in and Beyond the Galactic Center

Radio-wave scattering is enhanced dramatically for Galactic center sources in a region with radius >~ 15 arc min. Using scattering from Sgr A* and other sources, we show that pulse broadening for pulsars in the Galactic center is {\em at least} 6.3 \nu^{-4} seconds (\nu = radio frequency in GHz) and is most likely 50--200 times larger because the relevant scattering screen appears to be within the Galactic center region itself. Pulsars beyond---but viewed through---the Galactic center suffer even greater pulse broadening and are angularly broadened by <~ 2 {\em arc min}. Periodicity searches at radio frequencies are likely to find only long period pulsars and, then, only if optimized by using frequencies >~ 7 GHz and by testing for small numbers of harmonics in the power spectrum. The optimal frequency is $\nu ~ 7.3 GHz (\Delta_{0.1}P\sqrt{\alpha})^{-1/4}$ where \Delta_{0.1} is the distance of the scattering region from Sgr A* in units of 0.1 kpc, P is the period (seconds), and \alpha is the spectral index. A search for compact sources using aperture synthesis may be far more successful than searches for periodicities because the angular broadening is not so large as to desensitize the survey. We estimate that the number of {\em detectable} pulsars in the Galactic center may range from <= 1 to 100, with the larger values resulting from recent, vigorous starbursts. Such pulsars provide unique opportunities for probing the ionized gas, gravitational potential, and stellar population near Sgr A*.Comment: 13 pages, 4 PS figures, LaTeX and requires AASTeX macro aas2pp4, accepted by ApJ, also available as http://astrosun.tn.cornell.edu/SPIGOT/papers/pulsar/gc_psr.web