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Ethanol Induced Brain Lipid Changes in Mice Assessed by Mass Spectrometry
Alcohol abuse is a chronic disease
characterized by the consumption
of alcohol at a level that interferes with physical and mental health
and causes serious and persistent changes in the brain. Lipid metabolism
is of particular interest due to its high concentration in the brain.
Lipids are the main component of cell membranes, are involved in cell
signaling, signal transduction, and energy storage. In this study,
we analyzed lipid composition of chronically ethanol exposed mouse
brains. Juvenile (JUV) and adult (ADU) mice were placed on a daily
limited-access ethanol intake model for 52 days. After euthanasia,
brains were harvested, and total lipids were extracted from brain
homogenates. Samples were analyzed using high resolution mass spectrometry
and processed by multivariate and univariate statistical analysis.
Significant lipid changes were observed in different classes including
sphingolipids, fatty acids, lysophosphatidylcholines, and other glycerophospholipids