Study design.

<p>Study design.</p

Specific IgG1 production and inhibition of enzymatic activity of rSh28GST by sera of vaccinated volunteers.

<p>Adult human volunteers received three administrations of rSh28GST of 100 µg respectively at D0, D28 and D150. IgG1 production is expressed as mean +/− SEM of titers at each time. Titer was defined as the highest dilution yielding an absorbance three times above background. Enzymatic inhibition is expressed in % (histograms). Percentage of inhibition was calculated by the ratio GST activity after serum incubation to GST activity control. This value was considered significantly positive above 10%.</p

Anti-Sh28GST antibody levels in healthy adult volunteers after administration of Alum (comparator group).

a<p>Specific Ig antibody levels are expressed as the mean of individual titer (± SEM). Number of responders after vaccination is indicated in parentheses.</p>b<p>Day of comparator administration (D₀; D₂₈; D₁₅₀).</p

Main baseline laboratory results.

<p>Abbreviations: WBC, white blood cell count; PTT, partial thromboplastin time; ALT, alanine aminotransferase; AST, aspartate aminotransferase. Data are presented as mean ± standard deviation.</p>*<p>Local laboratory values. All other biological tests were in normal ranges.</p

Anti-Sh28GST antibody levels in healthy adult volunteers after administration of rSh28GST 100 µg.

a<p>Specific Ig antibody levels are expressed as the mean of individual titer (± SEM). Number of responders after vaccination is indicated in parentheses.</p>b<p>Day of Sh28GST administration (D₀; D₂₈; D₁₅₀).</p>*<p>Significantly different (P<0.05) compared to D₀ (comparison of mean using the Wilcoxon test).</p>**<p>Significantly different (P<0.05) compared to D₁₅₀ (comparison of mean using the Wilcoxon test).</p

Antibacterial Polyelectrolyte Micelles for Coating Stainless Steel

In this study, we report on the original synthesis and characterization of novel antimicrobial coatings for stainless steel by alternating the deposition of aqueous solutions of positively charged polyelectrolyte micelles doped with silver-based nanoparticles with a polyanion. The micelles are formed by electrostatic interaction between two oppositely charged polymers: a polycation bearing 3,4-dihydroxyphenylalanine units (DOPA, a major component of natural adhesives) and a polyanion (poly­(styrene sulfonate), PSS) without using any block copolymer. DOPA units are exploited for their well-known ability to anchor to stainless steel and to form and stabilize biocidal silver nanoparticles (Ag⁰). The chlorine counteranion of the polycation forms and stabilizes biocidal silver chloride nanoparticles (AgCl). We demonstrate that two layers of micelles (alternated by PSS) doped with silver particles are enough to impart to the surface strong antibacterial activity against gram-negative <i>E</i>. <i>coli</i>. Moreover, micelles that are reservoirs of biocidal Ag⁺ can be easily reactivated after depletion. This novel water-based approach is convenient, simple, and attractive for industrial applications

Clay and DOPA Containing Polyelectrolyte Multilayer Film for Imparting Anticorrosion Properties to Galvanized Steel

A facile and green approach is developed to impart remarkable protection against corrosion to galvanized steel. A protecting multilayer film is formed by alternating the deposition of a polycation bearing catechol groups, used as corrosion inhibitors, with clay that induces barrier properties. This coating does not affect the esthetical aspect of the surface and does not release any toxic molecules in the environment