12 research outputs found
Comparison of basal metabolic rates in rats subjected to salivariadenectomy or salivary duct ligation
The Relationship Between the Histology of the Thyroid and the Salivary Glands and the Incidence of Dental Caries in the Rat
Importance of Type of Diet During the Suckling Period on the Incidence of Experimental Dental Caries in Rats
Laboratory manual of biological chemistry for students of dentistry
Intended to accompany Textbook of biochemistry for students of dentistry, by J.C. Muhler.Bibliographical footnotes.Mode of access: Internet
Adsorption of water on Fe3O4(111) studied by photoelectron and thermal desorption spectroscopy
Salivary Gland Hypofunction in tyrosylprotein sulfotransferase-2 Knockout Mice Is Due to Primary Hypothyroidism
Background:Protein-tyrosine sulfation is a post-translational modification of an unknown number of secreted and membrane proteins mediated by two known Golgi tyrosylprotein sulfotransferases (TPST-1 and TPST-2). We reported that Tpst2-/- mice have mild-moderate primary hypothyroidism, whereas Tpst1-/- mice are euthyroid. While using magnetic resonance imaging (MRI) to look at the thyroid gland we noticed that the salivary glands in Tpst2-/- mice appeared smaller than in wild type mice. This prompted a detailed analysis to compare salivary gland structure and function in wild type, Tpst1-/-, and Tpst2 -/- mice.Methodology/Principal Findings:Quantitative MRI imaging documented that salivary glands in Tpst2-/- females were 30% smaller than wild type or Tpst1-/- mice and that the granular convoluted tubules in Tpst2-/- submandibular glands were less prominent and were almost completely devoid of exocrine secretory granules compared to glands from wild type or Tpst1-/- mice. In addition, pilocarpine-induced salivary flow and salivary α-amylase activity in Tpst2-/- mice of both sexes was substantially lower than in wild type and Tpst1-/- mice. Anti-sulfotyrosine Western blots of salivary gland extracts and saliva showed no differences between wild type, Tpst1-/-, and Tpst2-/- mice, suggesting that the salivary gland hypofunction is due to factor(s) extrinsic to the salivary glands. Finally, we found that all indicators of hypothyroidism (serum T4, body weight) and salivary gland hypofunction (salivary flow, salivary α-amylase activity, histological changes) were restored to normal or near normal by thyroid hormone supplementation.Conclusions/Significance:Our findings conclusively demonstrate that low body weight and salivary gland hypofunction in Tpst2-/- mice is due solely to primary hypothyroidism