2 research outputs found
Gold-Nanoparticle-Decorated Silica Nanorods for Sensitive Visual Detection of Proteins
We
report a rapid and highly sensitive approach based on gold-nanoparticle-decorated
silica nanorods (GNP-SiNRs) label and lateral-flow strip biosensor
(LFSB) for visually detecting proteins. Owing to its biocompatibility
and convenient surface modification, SiNRs were used as carriers to
load numerous GNPs, and the GNP-SiNRs were used as labels for the
lateral-flow assay. The LFSB detection limit was lowered 50 times
compared to the traditional GNP-based lateral-flow assay. Rabbit IgG
was used as a model target to demonstrate the proof-of-concept. Sandwich-type
immunoreactions were performed on the immunochromatographic strips,
and the accumulation of GNP-SiNRs on the test zone produced the characteristic
colored bands, enabling visual detection of proteins without instrumentation.
The quantitative detection was performed by reading the intensities
of the colored bands with a portable strip reader. The response of
the optimized device was highly linear for the range of 0.05–2
ng mL<sup>–1</sup>, and the detection limit was estimated to
be 0.01 ng mL<sup>–1</sup>. The GNP-SiNR-based LFSB, thus,
offered an ultrasensitive method for rapidly detecting trace amounts
of proteins. This method has a potential application with point-of-care
screening for clinical diagnostics and biomedical research