ZP_02147451 sequence coverage with non-tryptic, semi-tryptic, and tryptic peptides.

<p>The ZP_02147451 sequence is represented with its peptidase motif (residues 240 to 279) pointed out with purple stars. Peptides identified with the “no-enzyme” Mascot search are symbolized with a line underlining the sequence. Tryptic, semi-tryptic, and non-tryptic peptides are indicated in yellow, blue and red, respectively.</p

MS/MS spectrum of the semi-tryptic peptide [288–299] belonging to ZP_02147451.

<p>The MS/MS spectrum was acquired with a FT/FT procedure with an LTQ-Orbitrap XL hybrid mass spectrometer. The peptide sequence is shown on the top with the collision-induced fragmentation pattern. The <i>b</i> and <i>y</i> ions are shown in blue and red, respectively. The <i>y₁₁</i> di-charged ion is labeled in green.</p

SDS-PAGE of the exoproteome of <i>Phaeobacter strain DSM 17395</i>.

<p>Exoproteins were resolved by a long migration on a 10% SDS-PAGE and stained with SimplyBlue SafeStain (Invitrogen). Lane <b>M</b>: SeeBlue Plus2 molecular weight range marker (Invitrogen). Lane <b>E1</b>: <i>Phaeobacter strain DSM 17395</i> exoproteome grown in Marine Broth (20 µg). Lane <b>E2</b>: <i>Phaeobacter strain DSM 17395</i> exoproteome grown in Marine Broth (8 µg). The 55 kDa major component is indicated with an arrow.</p

Occurrence of basic residues in proteins from <i>Phaeobacter strain DSM 17395.</i>

<p>The graph reports the ratio Lysine (K) and Arginine (R) residues per protein length of all the proteins with length above 100 residues encoded by <i>Phaeobacter strain DSM 17395</i>. Proteins are symbolized by a blue triangle. RTX-like proteins and the other exoproteins detected by tandem mass spectrometry are represented by red and yellow squares, respectively. The ZP_02143988.1 (a), ZP_02147451.1 (b), ZP_02144693.1 (c), ZP_02146960.1 (d), ZP_02144235.1 (e), and ZP_02146514.1 (f) RTX-toxins are indicated. Only the ZP_02143988.1 RTX-like protein has been detected by mass spectrometry.</p

List of the first ten proteins identified from the 55<i>P. gallaeciensis</i> DSM¹.

1<p>Detected with at least three different peptides.</p><p>*Periplasmic component.</p

List of proteins identified in the 55/FT proteomic procedure¹.

1<p>Detected with at least three different peptides. The values are from a representative experiment.</p><p>*Periplasmic component.</p>$<p>EUF stands for Enzyme Unspecificity Factor, defined as the ratio number of unique peptides (no-enzyme MASCOT search) per number of unique peptides (trypsin MASCOT search).</p