15 research outputs found
Protection Elicited by Nasal Immunization with Recombinant Pneumococcal Surface Protein A (rPspA) Adjuvanted with Whole-Cell Pertussis Vaccine (wP) against Co-Colonization of Mice with <i>Streptococcus pneumoniae</i>
<div><p>A promising alternative vaccine candidate to reduce the burden of pneumococcal diseases is the protein antigen PspA (Pneumococcal surface protein A). Since concomitant colonization with two or more pneumococcal strains is very common in children, we aimed to determine if immunization with PspA would be able to control co-colonization. We evaluated nasal immunization with recombinant PspA (rPspA) in a model of co-colonization with two strains expressing different PspAs. Mice were immunized intranasally with rPspAs from clades 1 to 4 (rPspA1, rPspA2, rPspA3 or rPspA4) using whole-cell pertussis vaccine (wP) as adjuvant. Mice were then challenged with a mixture of two serotype 6B isolates St491/00 (PspA1) and St472/96 (PspA4). Immunization with rPspA1+wP and rPspA4+wP reduced colonization with both strains and the mixture of rPspA1+rPspA4+wP induced greater reduction than a single antigen. Immunization rPspA1+rPspA4+wP also reduced colonization when challenge experiments were performed with a mixture of isolates of serotypes 6B (PspA3) and 23F (PspA2). Furthermore, none of the tested formulations led to a pronounced increase in colonization of one isolate over the other, showing that the vaccine strategy would not favor replacement. Interestingly, the adjuvant wP by itself already led to some reduction in pneumococcal colonization, indicating the induction of non-specific immune responses. Anti-rPspA IgG was observed in serum, nasal wash (NW) and bronchoalveolar lavage fluid (BALF) samples, whereas animals inoculated with formulations containing the adjuvant wP (with or without rPspA) showed higher levels of IL-6 and KC in NW and increase in tissue macrophages, B cells and CD4<sup>+</sup>T cells in BALF.</p></div
Recovery of pneumococci from the nasopharynx of mice challenged with a mixture of two 6B strains–rPspA1 and rPspA4 mixture.
<p>Mice were immunized intranasally with two doses of the indicated formulations and challenged with a mixture of two 6B strains expressing different PspAs. Recovery of strains St491/00 (PspA1) (A) and St472/96 (PspA4) (B) in nasal washes performed 5 days after challenge is shown. * indicates statistical difference with saline (One-way ANOVA, Tukey’s Multicomparison Test—** P≤0.01).</p
Induction of anti-rPspA IgG serum antibodies.
<p>Mice were immunized intranasally with two doses of the indicated formulations and anti-rPspA1(A), anti-rPspA2 (B), anti-rPspA3 (C) and anti-rPspA4 (D) IgG in serum samples were detected by ELISA. * indicates statistical difference with saline (One-way ANOVA, Tukey’s Multicomparison Test—*** P≤0.001). Results are representative of two independent experiments.</p
Immunophenotyping of BALF cells.
<p>BALF samples were collected from mice immunized with the indicated formulations and infiltrated cells were immunophenotyped by flow cytometry. BALF was collected before (D0), at day 1 (D1) and at day 5 (D5) after intranasal challenge with a mixture of strains 23F OPKA (PspA2) and 6B OPKA (PspA3). Percentages of F4/80<sup>+</sup> CD11b<sup>+</sup> (A), F4/80<sup>+</sup> CD11c<sup>+</sup> (B), F4/80<sup>-</sup> B220<sup>+</sup> (C), F4/80<sup>-</sup> CD4<sup>+</sup> (D), F4/80<sup>-</sup> CD8<sup>+</sup> (E) and F4/80<sup>-</sup> Ly6G<sup>+</sup> (F) are shown. Differences with saline at the same day and differences within the same group at different days are shown (One-way ANOVA, Tukey’s Multicomparison Test—* P≤0.05; **P≤0.01, ***P≤0.001). Results are representative of two independent experiments.</p
Recovery of pneumococci from the nasopharynx of mice challenged with a mixture of serotype 23F and serotype 6B strains–rPspA1 and rPspA4 mixture.
<p>Mice were immunized intranasally with two doses of the indicated formulations and challenged with a mixture of serotype 23F and serotype 6B strains expressing different PspAs. The adjuvant wP was inoculated only in the first dose. Recovery of strains 23F OPKA (PspA2) (A) and 6B OPKA (PspA3) (B) in nasal washes performed 5 days after challenge is shown.</p
Recovery of pneumococci from the nasopharynx of mice challenged with a mixture of two 6B strains.
<p>Mice were immunized intranasally with two doses of the indicated formulations and challenged with a mixture of two 6B strains expressing different PspAs. Recovery of strains St491/00 (PspA1) (A) and St472/96 (PspA4) (B) in nasal washes performed 5 days after challenge is shown. * and <sup>#</sup> indicate statistical difference with saline and wP, respectively (One-way ANOVA, Tukey’s Multicomparison Test—* or <sup>#</sup> P≤0.05; ** P≤0.01; *** P≤0.001). Results are from two independent experiments.</p
Recovery of pneumococci from the nasopharynx of mice challenged with a mixture of serotype 23F and serotype 6B strains.
<p>Mice were immunized intranasally with two doses of the indicated formulations and challenged with a mixture of serotype 23F and serotype 6B strains expressing different PspAs. Recovery of strains 23F OPKA (PspA2) (A) and 6B OPKA (PspA3) (B) in nasal washes performed 5 days after challenge is shown. * indicates statistical difference with saline (One-way ANOVA, Tukey’s Multicomparison Test- * P≤0.05; **P≤0.01).</p
IL-6 and KC levels in nasal wash samples.
<p>NW samples were collected from mice immunized with the indicated formulations before (D0), at day 1 (D1) and at day 5 (D5) after intranasal challenge with a mixture of strains 23F OPKA (PspA2) and 6B OPKA (PspA3). IL-6 (A-C) and KC (D-F) were detected using a Luminex-based assay. * indicates statistical difference with saline (One-way ANOVA, Tukey’s Multicomparison Test—* P≤0.05; **P≤0.01, ***P≤0001). Results are representative of two independent experiments.</p
Phenotypic analyses of <i>L. interrogans</i> serovar Copenhageni after UV-C irradiation.
<p>(A) Relationship between survival after treatment with increasing doses of UV-C (left axis) and the mean size of the persistent cells (right axis). Cultures of bacteria were treated with increasing doses of UV-C, diluted in fresh medium and incubated during 24h in the dark. Surviving bacteria were counted and the frequency was calculated as the ratio of irradiated to non-irradiated cells. (B) Fluorescent microscopy of leptospires in cultures treated or not with UV-C, after staining with DAPI (1000x magnification). The bar represents 20 µm. (C) Frequencies of the size categories from treated and non-treated samples. Categories were determined by the size values (expressed in µm) that divided the non-irradiated culture in its four quartiles. All the data represent the average of three independent experiments.</p
Genomic and transcriptional organization of the <i>lexA1</i> region.
<p>(A) Schematic representation of the <i>lexA1</i> genomic region. The arrows indicate the direction of transcription. The fragments amplified by the primer pairs used for the RT-PCR analysis are indicated by numbered lines below the genes. (B) Composite image of agarose gels from resulting RT-PCR reactions, using either genomic DNA (DNA), RNA (RT-) or cDNA (RT+) as templates. The numbers refer to the respective fragments shown in (A).</p