One hundred of cells generations connect the resistance to drugs and the replicative aging mechanisms

There are researchs that reveal a relationship between acquired resistance to antineoplastic drugs and aging. Therefore, the identification of genes involved in senescence could have a potential therapeutic utility for the prediction and/or prevention of resistance to antineoplastic drugs. The aim of this work is to study the relationship between replicative aging and acquired resistance to Cisplatin and Bleomycin in the yeast Saccharomyces cerevisiae.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

Proteomic analysis in wild type and cisplatin resistant yeast strain of saccharomyces cerevisiae

Research carried out in oncology has allowed the identification and characterization of molecular markers of resistance to antineoplastic drugs. It supposes a great advance and allows the administration of customized chemotherapies to patients. The aim of this work is to carry out a proteomic analysis in the wild type strain and cisplatin resistant strain of Saccharomyces cerevisiae in order to look for possible markers of resistance, as well as the mechanisms involved.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

New markers of bleomycin resistance

Resistance to chemotherapy is one of the main problems of this type of therapy. Studies carried out determine the usefulness of predictive biomarkers to improve this treatment. The aim of this work is to carry out a proteomic analysis in the wild type strain and bleomycin resistant strain of Saccharomyces cerevisiae in order to look for possible markers of resistance, as well as the mechanisms involved.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

Mechanisms of cross resistance between cisplatin and bleomycin in saccharomyces cerevisiae

Tumor cells can simultaneously acquire resistance against a diverse group of drugs. This phenomenon is called pleotropic resistance or multidrug resistance. This mechanism could explain the tumor resistance observed in chemotherapy treatments with multiple agents. The aim of this work is to study possible mechanisms of cross resistance between Cisplatin and Bleomycin in Saccharomyces cerevisiae.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

Resistance to bleomycin increases the chronological life of cells

The identification of genes involved in chronological aging could have a potential utility as molecular markers in the chemotherapy treatment of cancer. The aim of this work is to study the relationship between the chronological aging and the resistance to Bleomycin for the purpose of establish the basic interactions between these phenomenons for further investigation of molecular markers.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

Resistance acquisition after seven cycles of in vitro bleomycin exposure

Bleomycin is an antineoplastic drug that causes DNA damage by intra- and inter-strand crosslink and DNA strand breaks. This drug is used to treat several types of cancer. The aim of this work is to study the effect of Bleomycin on S. cerevisiae cells and select bleomycin-resistant cells to study the process of resistance acquisition to establish new protocols in in vitro drugs exposure.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

Relationship between chronological aging and acquired resistance to cisplatin in the yeast saccharomyces cerevisiae

The Sir2 gene is associated with an increase in longevity in yeasts, worms, flies and rodents. The human homolog, Sirt1, is also involved in longevity, by inhibiting cellular senescence. Studies carry out in tumor cells of neuroblastoma, osteosarcoma, breast and ovary, resistant and sensitive to antineoplastic drugs, suggest that the increase of Sirt1 expression may represent a general phenomenon associated with resistance to chemotherapy, independent of cell type or drug used to induce resistance. Thus, after analyzing tumor biopsies after treating patients with chemotherapy, high amounts of Sirt1 have been observed. The aim of this work is to study the relationship between chronological aging and acquired resistance to Cisplatin in the yeast Saccharomyces cerevisiae.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

Selection of resistance to cisplatin in saccharomyces cerevisiae as a model for antineoplastic drugs studies

The budding yeast (S. cerevisiae) is an excellent eukaryotic model to study the antineoplastic drugs effects, due to the well-characterized metabolic and genetic characteristics and the conserved similarity in molecular mechanisms with other species including human cells. The aim of this work is to study the effect of cisplatin on S. cerevisiae cells and select cisplatin-resistant cells of this organism as a model of study of resistance to antineoplastic drugs.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

Anaerobic co-digestion of municipal sewage sludge and fruit/vegetable waste: effect of different mixtures on digester stability and methane yield

There are different options for the management of fruit and vegetable wastes (FVWs), but the most environmental-friendly is the anaerobic digestion, because it allows an optimum recovery of materials and energy from the two by-products: biogas and digestate. Nevertheless, in many cases there are economic and technical problems that cause the selection of other alternatives. Frequently these wastes are produced in large quantities but only during few weeks of the year. In these cases, this is the most important economic problem, because large digesters that would be used only for short time periods every year would be required. In addition, a close control of the pH of the digester is required for this kind of residues, for which the hydrolysis is usually faster than the methanogenesis, so large concentrations of fatty acids should be prevented to maintain the adequate pH value for anaerobic digestion that should be neutral or slightly alkaline. Both problems can be simultaneously overcome by the co-digestion with other residues that are produced throughout the year. Among the benefits of co-digestion, one of the most important is the improvement in the feedstock characteristics, since it may allow a more equilibrate composition resulting in a better performance of the digester in treatment capacity, and a better quality of biogas and digestate. The co-digestion with other substrates with a complementary composition that are produced throughout the year and that are already managed by anaerobic digestion is probably the optimum management option. For these cases, if the existing anaerobic digester is oversized and allows the introduction of additional volumes of wastes, the mean retention time of the digestate should be maintained. Therefore, the anaerobic co-digestion of fruit and vegetable waste (FVW) and municipal sewage sludge (MSS) under mesophilic condition and a constant hydraulic retention time (20 d) is studied. The effects on digester performance of the FVW:MSS ratio and the organic loading rate (OLR) were examined. The OLR is the mass of volatile solids fed per volume of digestate and day.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech