1 research outputs found
Intestinal Anti-inflammatory Effects of Oligosaccharides Derived from Lactulose in the Trinitrobenzenesulfonic Acid Model of Rat Colitis
Intestinal microbiota modulation
is becoming an interesting approach
to manage inflammatory bowel disease and can be achieved by the administration
of prebiotics. Previous studies showed the intestinal anti-inflammatory
effects of the prebiotic lactulose. The aim of the present study was
to test the preventative effects of oligosaccharides derived from
lactulose with prebiotic properties (OsLu) in the trinitrobenzenesulfonic
acid model of rat colitis and compare them with those of lactulose.
Both treatments modified bacterial profile in intestinal contents,
increasing the bifidobacteria and lactobacilli counts and up-regulating
the production of short-chain fatty acids, although OsLu generated
a larger amount. OsLu also inhibited to a greater extent different
pro-inflammatory markers such as interleukins (IL) 1, 6, 12, and 23
and chemokines (MCP-1 and CINC-1). However, both prebiotics equally
restored colonic epithelial integrity, evaluated both with a histological
score (OsLu, 9.8 ± 2.2; and lactulose, 12.1 ± 2.1, vs colitic
control, 27.3 ± 3.3) and by measuring several key proteins of
the mucosal barrier (MUC-2, MUC-3, and TTF-3). OsLu effect was also
associated with an inhibition of iNOS expression and a reduction of
Th17 cell activity in the inflamed tissue that facilitated the intestinal
mucosa barrier recovery. In conclusion, OsLu showed a better anti-inflammatory
profile than lactulose in this model of experimental colitis