Alignment of the Walker A and B motifs in ParA proteins.

<p>Alignment of the Walker A and B motifs in ParA proteins.</p

Genome organization of mycobacteriophage First.

<p>The scheme shows the gene annotation of mycobacteriophage First, the dashed arrow at gp25 represents the (−1) frameshift that generates gp 24 and gp25.</p

PFGE of DNA of mycobacteriophages Jolie, Mine, and 41HC.

<p>DNA of mycobacteriophages Jolie, Mine, and 41HC (not heated (NH) or heated (H) at 80°C for 10 min and cooled down in ice immediately prior to loading. DNA molecular weight (kb) on the standards is indicated at the right.</p

Phylogenetic analysis of mycobacteriophage partition proteins present in plasmids (pTAR, pTiC58, F, pRA2), non-mycobacterial bacteriophages (P1, N15, LE1) and mycobacteriophages.

<p>The neighbourjoining (NJ) tree was obtained with the MEGA5 software using an output from an alignment in ClustalW; bootstrap values from 1000 iterations are shown for ParA (A) and ParB (B) proteins.</p

Inheritance of “temperate” mycobacteriophages.

<p>(#)- Genome sequence not assembled, so no accurate position for <i>par</i>A and <i>par</i>B genes, tandem repeat sequences (TRs) are listed for 21AM and 21AS mycobacteriophages.(*) N/A- Not applicable; (a)-TRs upstream <i>par</i>A gene are possible centromeres, thus only TRs found at this position are reported; (b)- Not detected bioinformatically; (^§)- Bioinformatically detected.</p

Genometrics of the novel mycobacteriophages isolated.

<p>Genometrics of the novel mycobacteriophages isolated.</p

Partitioning loci location in mycobacteriophages.

<p>Schematic representations of the mycobacteriophage genomes showing the location of putative <i>par</i>A and <i>par</i>B genes and sequences of tandem repeats (TR) functioning as putative centromers. Genes transcribed leftward are indicated. RedRock is included as a representative of <i>par</i>A/<i>par</i>B containing mycobacteriophages as published by Hatfull <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0056384#pone.0056384-Hatfull3" target="_blank">[31]</a>.</p