Comparison of genomes of ERPV and ECTV-Nav and ECTV-Mos.

a<p>Genome sizes are from the first nt of the left CRS to the nt before the right CRS.</p>b<p>The ORF number includes the homolog of O3, which was not originally annotated in ECTV-Nav or ECTV-Mos.</p

Phylogenetic tree of orthopoxviruses including ERPV.

<p>Concatenated sequences of 96 ORFs conserved in each viral genome were used to perform the analysis. ERPV branches from the same node as ECTV.</p

Unassigned ORFs with homology to CPXV proteins.

a<p>Similar in ECTV-Mos.</p>b<p>Similar in ECTV-Nav.</p>c<p>Missing from ECTV-Mos. Others are identical in the three genomes.</p><p>Abbreviation: aa, amino acids.</p

Genome map of ERPV and comparison to ECTV-Naval.

<p>Left (LITR) and right (RITR) inverted terminal repeats are indicated by deep blue arrows. ORFs are in yellow and numbered from left to right with the direction of transcription indicated by the arrow. Direct repeats (DRs) are indicated in light blue. Single nucleotide polymorphisms (SNPs) are gold; insertions and deletions are indicated in red and purple, respectively, with single nt and larger changes by a thin oval and a diamond, respectively. Asterisks signify mutations that affect the predicted amino acid sequence.</p

Assembly of contigs and gap closure.

<p>(A) Five contigs were assembled de novo using 159,077 sequence reads generated by pyrosequencing, providing an estimated coverage of 60X with 5 gaps. (B) The gaps were filled by PCR and Sanger sequencing. Blue arrows indicate positions of primers used for PCR. Gaps 2 and 5 contained direct repeats (DRs) necessitating synthesis and sequencing of additional internal PCR fragments. DRI contains a 69 bp sequence repeated 2.3X; DRII contained an 85 bp sequence repeated 10.4X; and DRIII contained a 25 bp sequence repeated 7.0X. The non-repetitive I (NRI) and NRII sequences flank DRI. ORFs are indicated by numbered yellow arrows.</p

Representation of an orthopoxvirus genome.

<p>A typical genome consisting of a single dsDNA molecule with a concatemer resolution sequence (CRS), sets of direct repeats (DRI and DRII) and a hairpin loop on each inverted terminal repeat (ITR) is shown.</p