Positivity and amplification success of two RABV fecal samples declined at dilutions exceeding 1:10.

Dilutions positive for rabies virus are in bold; LN34 is mean Ct over three replicates for the LN34 assay, with standard deviation in parentheses; BA is Ct for the β-actin assay; # Amp is the number of successful amplifications; and, the positive control was a synthetic sequence provided by the Center for Disease Control. RABV fecal 1 was from a Tadarida brasiliensis and RABV fecal 2 was from an Eptesicus fuscus.</p

Positivity of fecal samples harvested from ten rabid <i>Parastrellus hesperus</i> carcasses and tested at time 0 and after 24 hours at ambient conditions.

Positivity of fecal samples harvested from ten rabid Parastrellus hesperus carcasses and tested at time 0 and after 24 hours at ambient conditions.</p

Using the LN34 assay, rabies virus was detected in the brainstem, mouth swab, and feces of rabid bat carcasses.

Values are Ct means of three replicates, with standard deviations in parentheses. BA = β-actin.</p

Standard curve regression coefficients, quantification reliability [PCR efficiency and limit of quantification (LOQ)], and sensitivity estimates [limit of detection (LOD) via probit modeling].

Estimates of LOD are predicted assuming n replicates per sample (i.e., effective LOD), with 95% confidence intervals in brackets. The bolded row highlights the effective LOD reflecting the proposed number of qPCR replicates to run for regular screening.</p

qPCR results for sample type comparison, time tests, and pooling experiment.

qPCR results for sample type comparison, time tests, and pooling experiment.</p

Positivity of fecal samples harvested from rabid bat carcasses and tested at 24, 48, and 72 hours at ambient conditions.

At 72 hours, at least one replicate was positive for 4 of 6 samples (a) and all three replicates were positive for 3 of 6 samples (b). Bat species tested included Eptesicus fuscus (n = 1), Tadarida brasiliensis (n = 3), Antrozous pallidus (n = 1), and Lasiurus xanthinus (n = 1).</p