Connecting Green Infrastructure with Transportation Planning

The development of green infrastructure planning is increasingly espoused by landscape, conservation, and metropolitan planners as an effective way to create a network of ecological functionality in regions that are otherwise highly impacted by anthropogenic changes. The most common condition is one of expanding metropolitan growth transforming cultivated and natural landscapes into developed ones. Despite its promise, several critical problems arise with green infrastructure planning. We will consider three. The first is that the definition of green infrastructure within the public realm remains highly variable if not embryonic. The second is green infrastructure is a long-term investment whose effects will not be able to be measured until years after implementation, yet there are very few cases of green infrastructure planning that have actually resulted in adopted and implemented plans, which can be used as models of success. And third, although the public benefits of green infrastructure are positive, other forms of regional scale planning have more dominant influence and impacts, most notably transportation planning. Concerning this last point, our thesis is that green infrastructure and transportation infrastructure have important interactions. Furthermore, transportation infrastructure potentially degrades green infrastructure networks through fragmentation, ecosystem impairment, and loss of connectivity. Mitigating the impacts of transportation infrastructure could potentially be an important asset to green infrastructure planning. Coordination of transportation and green infrastructure holds promise for sustainable development across regional landscapes. We address this thesis by assessing the landscape significance of both green and transportation infrastructure planning and presenting a case study from Maryland, USA. The case study indicates that there are numerous logical connections between the two types of plans, some of them conflicting. However, it also indicates that there is much research still necessary on green infrastructure, especially in the areas of implementation and efficacy

Perceived quality of life impacts and tourism-related stress

Tourism has many impacts on residents’ quality of life. One outcome that can result from these impacts is the experience of stress. Individuals who experience stress are at increased risk for mental and physical illnesses. This paper examines the relationship between residents’ perceived impacts of tourism on their quality of life and their experience of tourism related stress. Paper surveys were completed by 247 residents of the Hawai’ian island of Oahu, a heavily developed tourism destination that is economically reliant on the tourism industry. A logistic regression model revealed that clean air and water, crowding and congestion, the preservation of residents’ way of life, the value of residents’ house and/or land, and plenty of festivals, fairs, and museums, were all significantly related to the experience of tourism related stress. Tourism decision makers can address tourism quality of life impacts in these specific areas to help mitigate tourism related stress

Negligence at the Breach: Information Fiduciaries and the Duty to Care for Data

Personal data is a cost of admission for much of modern life. Employers, tech companies, advertisers, information brokers, and others collect huge quantities of data about us all. Yet outside of a few highly-regulated industries, American companies face few legal restrictions on how they manage and use that data. Until now, individuals have had very limited remedies when their data is stolen from data collectors. But change is afoot. In a significant recent decision, the Pennsylvania Supreme Court took a consequential step holding that entities collecting personal data owe a duty of reasonable care to protect data subjects against harm. This tort decision left a critical question unresolved. What is “harm” in the context of privacy? What is it exactly that data collectors must protect data subjects against? This Article takes one state’s doctrinal move as a jumping-off point to consider a question of immense national importance—how to apply common law negligence principles in cases involving the disclosure and misuse of personal data, and specifically, what a “duty to care” means in the unsettled realm of privacy law. Building off Jack Balkin’s work, this Article proposes that fiduciary law offers an appealing framework for conceptualizing privacy harms and the corresponding responsibilities of the entities who are collecting our data. In doing so, it begins the conversation of how tort law can take a central place in protecting individuals when data holders betray their trust

Beyond Detection: Investing in Practical and Theoretical Applications of Emotion + Visualization

Emotion is a dynamic variable that modulates how we perceive, reason about, and interact with our environment. Recent studies have established that emotion’s influence carries to data analysis and visualization, impacting performance in ways both positive and negative. While we are still in the infancy of understanding the role emotion plays in analytical contexts, advances in physiological sensing and emotion research have raised the possibility of creating emotion-aware systems. In this position paper, we argue that it is critical to consider the potential advances that can be made even in the face of imperfect sensing, while we continue to address the practical challenges of monitoring emotion in the wild. To underscore the importance of this line of inquiry, we highlight several key challenges related to detection, adaptation, and impact of emotional states for users of data visualization systems, and motivate promising avenues for future research in these areas

Intensification of MVA and influenza virus production through high-cell-density cultivation approaches

Background. Unlike production of recombinant proteins, continuous production of viral vaccines at high cell densities (HCD) is often constrained by a decrease in cell-specific virus yields, early host cell lysis during virus propagation and limited virus recovery from culture broth. Nevertheless, advanced fed-batch [1] and perfusion strategies can be applied to achieve high-yield virus production processes. In this study, the development of a semi-continuous process for the production of the modified vaccinia Ankara virus isolate MVA-CR19 and influenza virus A/PR/8/34 (H1N1) in HCD cultivations of the suspension cell line AGE1.CR.pIX (ProBioGen AG, Berlin) is presented. Methods. Depending on the required scale, high cell concentrations (~ 50×106 cells/mL) were achieved either through medium renewal by periodic centrifugation (semi-perfusion) in 50 mL cultivations or using an alternating tangential flow (ATF) perfusion system for 1 L bioreactors. Process development and optimization comprised three phases: 1) assessment of different fed-batch and medium exchange strategies for the propagation of MVA-CR19 or influenza A/PR/8/34 viruses in 50 mL cultivations; 2) scale-up and process optimization of the selected high-yield process strategy to a 1 L bioreactor with the ATF system, and 3) integration of a one-step purification process using magnetic sulfated cellulose particles (MSCP). For both viruses, conventional batch cultivation (no addition/medium exchange after infection) was compared with processes applying fed-batch, periodic medium exchange and the combination of both during virus propagation. Results. Perfusion and semi-perfusion at a feeding rate of 0.05 nL/cell×d was suitable to propagate AGE1.CR.pIX cells above 60×106 cells/mL with neither limitation nor overload of nutrients. For infections at 50 mL scale, the application of a combined strategy comprising an initial fed-batch phase followed by a periodic virus harvest phase resulted in the highest product yield with a more than 10-fold increase in virus particles concentration compared to the conventional batch processes operated at 4 to 8×106 cells/mL [2]. Additionally, a 3-fold increase in both cell-specific yield (virus particles/cell) and volumetric productivity (virus particles/L×d) could be obtained. Comparable yields were observed when up-scaling to a 1 L bioreactor using an ATF-system, even when virus particles were retained within the bioreactor. Further selection of the optimal pore size of the ATF membrane allowed semi-continuous harvesting of the produced viruses and its purification with MSCPs with a recovery from 30 to 50%. In all cases, cell-specific yields and volumetric productivities reached their maxima at 72 h post-infection, indicating that the process should be stopped at that time point. Conclusion. Compared to conventional batch processes, the developed HCD process offers significantly higher productivities including the option to integrate a one-step purification process in a semi-continuous mode. Overall, the results show that there is a great potential for semi-continuous HCD processes for the production of viral vaccines in larger scales, which could support efforts towards the establishment of continuous vaccine manufacturing. References. 1. Pohlscheidt, M., et al., Development and optimisation of a procedure for the production of Parapoxvirus ovis by large-scale microcarrier cell culture in a non-animal, non-human and non-plant-derived medium. Vaccine, 2008. 26(12): p. 1552-65. 2. Lohr, V., et al., New avian suspension cell lines provide production of influenza virus and MVA in serum-free media: studies on growth, metabolism and virus propagation. Vaccine, 2009. 27(36): p. 4975-82

Galaxy Zoo: Disentangling the Environmental Dependence of Morphology and Colour

We analyze the environmental dependence of galaxy morphology and colour with two-point clustering statistics, using data from the Galaxy Zoo, the largest sample of visually classified morphologies yet compiled, extracted from the Sloan Digital Sky Survey. We present two-point correlation functions of spiral and early-type galaxies, and we quantify the correlation between morphology and environment with marked correlation functions. These yield clear and precise environmental trends across a wide range of scales, analogous to similar measurements with galaxy colours, indicating that the Galaxy Zoo classifications themselves are very precise. We measure morphology marked correlation functions at fixed colour and find that they are relatively weak, with the only residual correlation being that of red galaxies at small scales, indicating a morphology gradient within haloes for red galaxies. At fixed morphology, we find that the environmental dependence of colour remains strong, and these correlations remain for fixed morphology \textit{and} luminosity. An implication of this is that much of the morphology--density relation is due to the relation between colour and density. Our results also have implications for galaxy evolution: the morphological transformation of galaxies is usually accompanied by a colour transformation, but not necessarily vice versa. A spiral galaxy may move onto the red sequence of the colour-magnitude diagram without quickly becoming an early-type. We analyze the significant population of red spiral galaxies, and present evidence that they tend to be located in moderately dense environments and are often satellite galaxies in the outskirts of haloes. Finally, we combine our results to argue that central and satellite galaxies tend to follow different evolutionary paths.Comment: 19 pages, 18 figures. Accepted for publication in MNRA