Confirmation of PMCA4 knockout in the kidney.

<p>(<b>A</b>) Renal relative mRNA expression levels (corrected for GAPDH) of PMCA4 in wild type (WT, n = 10), heterozygous (HZ, n = 7) and knockout (KO, n = 10) mice. Data represents mean ± S.E.M. *P<0.05 compared to WT. Real-time PCR products were subjected to 2% (w/v) agarose gel electrophoresis. (<b>B-D</b>) Representative immunofluorescence image for PMCA4 expression in mouse kidney cortex of wild type (<b>B</b>) and PMCA4 knockout mice (<b>C</b>). (<b>D</b>) Negative control without the primary antibody.</p

Calcium Extrusion Pump PMCA4: A New Player in Renal Calcium Handling? - Fig 5

<p><b>PMCA4 ablation does not affect serum PTH but increases FGF23 serum levels</b> (<b>A</b>) Serum PTH levels were measured in wild type (WT, n = 10), heterozygous (HZ, n = 7) and PMCA4 knockout mice (KO, n = 10). Relative mRNA expression levels (corrected for GAPDH) of the renal vitamin D activating enzyme Cyp27b1 (<b>B</b>) and vitamin D degrading enzyme Cyp24a1 (<b>C</b>). (<b>D</b>) Serum FGF23 levels were significantly higher in KO mice compared to WT. Data represents mean ± S.E.M, *P<0.05 compared to WT.</p

Effects of the combinatory application of omeprazole and 10% inulin enriched diets on electrolytes.

<p>Serum Ca²⁺ concentration (A), 24 h urinary Ca²⁺ excretion (B), 24 h fecal Ca²⁺ excretion, serum K⁺ concentration (D) and serum Na⁺ concentration (E). Significant differences compared to control are indicated by * with <i>P</i> < 0.05, significant differences between the omeprazole-treated groups are annotated by # with <i>P</i> < 0.05. Bars represent means ± SEM, with n = 10 mice per group.</p

No effect of PMCA4 ablation on mRNA expression of Ca²⁺-related genes in duodenum.

<p>TRPV6 (<b>A</b>), NCX1 (<b>B</b>), PMCA1 (<b>C</b>) and calbindin-D_9k (CaBP_9k, <b>D</b>) relative mRNA expression levels (corrected for GAPDH) were analyzed in the duodenum of wild type (WT, n = 10), heterozygous (HZ, n = 7) and PMCA4 knockout mice (KO, n = 10). Data represents mean ± S.E.M.</p

Effects of omeprazole treatment and diets enriched with 10% (w/w) inulin on mRNA levels of PPIH candidate genes.

<p>Expressional levels of <i>Trpm6</i> and <i>Atp12a</i>, encoding the nongastric H⁺,K⁺-ATPase in cecum (A + D), colon (B + E) and kidney (C + F) of C57BL/6J mice compared to control (corrected for <i>Gapdh</i> expression). Significant differences compared to control are indicated by * with <i>P</i> < 0.05, significant differences between the omeprazole-treated groups are annotated by # with <i>P</i> < 0.05. All values represent means ± SEM, with n = 10 mice per group.</p

Metabolic parameters of the animals.

<p>The results are expressed as means ± SEM (n = 10). Significant differences between the two omeprazole-treated groups are indicated by</p><p>^# with <i>P</i> < 0.05. Mice have been fed a control diet or a diet containing 10% (w/w) oligofructose enriched inulin fibers. Treated mice received 20 mg/kg bodyweight omeprazole.</p><p>Metabolic parameters of the animals.</p