Selective autophagy in the Hepatitis C Virus NS5A degradation

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Interferon-inducible proteoglycan in the regulation of virus infection

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Hydrological Modeling Approach Using Radar-Rainfall Ensemble and Multi-Runoff-Model Blending Technique

The purpose of this study is to reduce the uncertainty in the generation of rainfall data and runoff simulations. We propose a blending technique using a rainfall ensemble and runoff simulation. To create rainfall ensembles, the probabilistic perturbation method was added to the deterministic raw radar rainfall data. Then, we used three rainfall-runoff models that use rainfall ensembles as input data to perform a runoff analysis: The tank model, storage function model, and streamflow synthesis and reservoir regulation model. The generated rainfall ensembles have increased uncertainty when the radar is underestimated, due to rainfall intensity and topographical effects. To confirm the uncertainty, 100 ensembles were created. The mean error between radar rainfall and ground rainfall was approximately 1.808–3.354 dBR. We derived a runoff hydrograph with greatly reduced uncertainty by applying the blending technique to the runoff simulation results and found that uncertainty is improved by more than 10%. The applicability of the method was confirmed by solving the problem of uncertainty in the use of rainfall radar data and runoff models

The B cell death function of obinutuzumab-HDEL produced in plant (<i>Nicotiana benthamiana</i> L.) is equivalent to obinutuzumab produced in CHO cells

<div><p>Plants have attracted attention as bio-drug production platforms because of their economical and safety benefits. The preliminary efficacy of ZMapp, a cocktail of antibodies produced in <i>N</i>. <i>benthamiana</i> (<i>Nicotiana benthamiana</i> L.), suggested plants may serve as a platform for antibody production. However, because the amino acid sequences of the Fab fragment are diverse and differences in post-transcriptional processes between animals and plants remain to be elucidated, it is necessary to confirm functional equivalence of plant-produced antibodies to the original antibody. In this study, Obinutuzumab, a third generation anti-CD20 antibody, was produced in <i>N</i>. <i>benthamiana</i> leaves (plant-obinutuzumab) and compared to the original antibody produced in glyco-engineered Chinese hamster ovary (CHO) cells (CHO-obinutuzumab). Two forms (with or without an HDEL tag) were generated and antibody yields were compared. The HDEL-tagged form was more highly expressed than the non-HDEL-tagged form which was cleaved in the N-terminus. To determine the equivalence in functions of the Fab region between the two forms, we compared the CD20 binding affinities and direct binding induced cell death of a CD20-positive B cells. Both forms showed similar CD20 binding affinities and direct cell death of B cell. The results suggested that plant-obinutuzumab was equivalent to CHO-obinutuzumab in CD20 binding, cell aggregation, and direct cell death via binding. Therefore, our findings suggest that Obinutuzumab is a promising biosimilar candidate that can be produced efficiently in plants.</p></div