Comparison of the transcript levels of selected genes estimated by transcriptome statistics and real-time qPCR.

<p>(<b>A</b>) The cpm values of histone H2A (H2A), catenin ß, Mos, Y-Box transcription factor, and 40S ribosomal protein S2 (Ribo S2) were normalized with that of the internal control gene. (<b>B</b>) Quantitation of the same transcripts in mature eggs (Mat), zygotes (Zyg), and early embryos (EE) by real-time qPCR. The difference of the bars marked with distinct alphabetic characters (e.g. <i>a</i> versus <i>b</i>) is considered statistically significant. P<0.01 (<i>post hoc</i> test) in all cases except the ones indicated with the asterisks (P<0.05). Histograms with error bars indicate mean ± standard deviation.</p

Comparison of the <i>A</i>. <i>aranciacus</i> reference transcriptome with the existing genetic sequence databases.

<p>Comparison of the <i>A</i>. <i>aranciacus</i> reference transcriptome with the existing genetic sequence databases.</p

Transcripts encoding putative enzymes and ion channels involved in intracellular calcium signaling.

<p>Transcripts encoding putative enzymes and ion channels involved in intracellular calcium signaling.</p

Families of cyclin and cyclin-dependent kinase.

<p>Families of cyclin and cyclin-dependent kinase.</p

Comparison of the transcript population in the cells of the four different stages on the basis of pathway annotation.

<p>Transcripts were classified with respect to the metabolic pathways to which their deduced proteins contribute. The occurrence frequencies of the major pathways were plotted in reference to the net annotated transcripts in the cells of four stages (percentage, Y-axis). The three plots show the three different levels of pathways as in UniPathway: level 1 (<b>A</b>), level 2 (<b>B</b>), level 3 (<b>C</b>).</p

Quantitation of the transcripts encoding proteins of the cyclin family.

<p>(<b>A</b>) Abundance of the transcripts encoding distinct cyclins in mature eggs. Data were presented both in CPM (green bars) and in RPKM (brown bars). (<b>B</b>) Relative expression levels of cyclin family in the GV-stage oocytes (GV), mature eggs (Mat), zygotes (Zyg) and early embryos (EE) estimated by real-time qPCR. Data were normalized with the values of the internal control transcript and plotted on a logarithmic scale as described in Materials and methods. Histogram and data points with error bars indicate mean ± standard deviation (n = 4).</p

Comparison of the transcript population in the cells of the four different developmental stages based on the occurrence frequency of GO classes.

<p>(<b>A</b>) Morphology of the cells at the different developmental stages: GV-stage oocytes, mature eggs, zygotes (20 min post-fertilization), and the early embryos (4 h and 20 min post-fertilization). (<b>B</b>) Comparison of the four stages on the basis of the three GO domains: biological processes (BP), molecular functions (MF) and cellular components (CC). The numbers on the Y-axis represent the percentage of the transcripts belonging to the labeled category in reference to the net annotated transcript population at the given stage.</p