1 research outputs found
Profiling Sulfur(VI) Fluorides as Reactive Functionalities for Chemical Biology Tools and Expansion of the Ligandable Proteome
Here, we report a comprehensive profiling of sulfur(VI)
fluorides
(SVI-Fs) as reactive groups for chemical biology applications.
SVI-Fs are reactive functionalities that modify lysine,
tyrosine, histidine, and serine sidechains. A panel of SVI-Fs were studied with respect to hydrolytic stability and reactivity
with nucleophilic amino acid sidechains. The use of SVI-Fs to covalently modify carbonic anhydrase II (CAII) and a range
of kinases was then investigated. Finally, the SVI-F panel
was used in live cell chemoproteomic workflows, identifying novel
protein targets based on the type of SVI-F used. This work
highlights how SVI-F reactivity can be used as a tool to
expand the liganded proteome