Selective restoration of TH in virally-rescued DD mice.

<p><i>A–E)</i> Immunohistochemistry (IHC) results for a control mouse. <i>A)</i> Tyrosine hydroxylase (TH) stain in the nucleus accumbens (NAc). <i>B)</i> 40x magnification of TH stain. <i>C)</i> Stain for dopamine transporter (DAT) in NAc. <i>D)</i> Merged image of TH and DAT stain showing extensive overlap of the two signals. <i>E)</i> TH stain in basolateral amygdala (BLA) shown at 20x. <i>F–J)</i> IHC results for a non-rescued DD mouse. <i>F)</i> Complete absence of TH in NAc. <i>G)</i> 40x magnification demonstrating lack of TH in NAc. <i>H)</i> DAT stain. <i>I)</i> Merged image of TH and DAT illustrating lack of TH in the NAc. <i>J)</i> TH in BLA (20x) is almost undetectable. <i>K–O)</i> Representative IHC from NAc-rescued DD mouse. <i>K)</i> TH was largely restored in the NAc. <i>L)</i> 40x magnification illustrating rescue of TH. <i>M)</i> DAT staining in NAc-rescued DD mouse. <i>N)</i> Merged image of TH and DAT illustrating large extent of TH restoration in NAc. <i>O)</i> TH in BLA (20x) remains at low, non-injected levels. <i>P–T)</i> Representative IHC from DD mouse injected into the NAc and BLA. <i>P)</i> Robust restoration of TH to the NAc. <i>Q)</i> 40x magnification demonstrating TH rescue. <i>R)</i> DAT staining. <i>S)</i> Merged image of TH and DAT showing extensive restoration of TH to NAc. <i>T)</i> TH is restored to higher levels in NAc and BLA rescued DD mice.</p

HPLC Quantification of DA, NE, and DA metabolites.

<p>DA, dopamine; HVA, homovanillic acid; DOPAC, 3.4-dihydroxyphenylacetic acid; 3-MT, 3-methoxytyramine. Data presented as means ± SEM.</p