1 research outputs found
Generation and Characterization of Standardized Forms of Trehalose Dihydrate and Their Associated Solid-State Behavior
Trehalose
dihydrate is a nonreducing disaccharide which has generated
great interest in the food and pharmaceutical industries. However,
it is well recognized that considerable batch to batch variation exists
for supposedly identical samples, particularly in terms of the thermal
response. In this investigation, two standardized forms of trehalose
dihydrate were generated using two distinct crystallization pathways.
The two batches were characterized using scanning electron microscopy,
X-ray powder diffraction, and FTIR. The thermal responses of the two
forms were then studied using modulated temperature differential scanning
calorimetry (MTDSC) and thermogravimetric analysis (TGA). In particular,
we describe the technique of quasi-isothermal MTDSC as a means of
studying the change in equilibrium heat capacity as a function of
temperature. Finally, variable temperature FTIR was utilized to assess
the change in bonding configuration as a function of temperature.
SEM revealed significant differences in the continuity and grain structure
of the two batches. The TGA, MTDSC, and quasi-isothermal MTDSC studies
all indicated significant differences in the thermal response and
water loss profile. This was confirmed using variable temperature
FTIR which indicated differences in bond reconfiguration as a function
of temperature. We ascribe these differences to variations in the
route by which water may leave the structure, possibly associated
with grain size. The study has therefore demonstrated that chemically
identical dihydrate forms may show significant differences in thermal
response. We believe that this may assist in interpreting and hence
controlling interbatch variation for this material