MOESM1 of Biomarkers allow detection of nutrient limitations and respective supplementation for elimination in Pichia pastoris fed-batch cultures

Additional file 1: Figure S1. Mean expression values (microarray data) of the carboxypeptidase B gene during the methanol and glucose fed-batch process. Table S2. Description of biological function of marker genes adapted from Saccharomyces Genome Database (SGD). Table S3. qPCR primer sequences. Figure S4. Principal component analysis (variable factor map; F2 vs. F1) of the methanol fed-batch (1 h, 15 h, 27 h, 41 h, 53 h and 67 h after starting the methanol fed-batch) referred to the glycerol fed-batch (log2 fold change). Figure S5. Principal component analysis (variable factor map; F3 vs. F2) of the methanol fed-batch (1 h, 15 h, 27 h, 41 h, 53 h and 67 h after starting the methanol fed-batch) referred to the glycerol fed-batch (log2 fold change). Figure S6. Principal component analysis (variable factor map; F2 vs. F1) of the glucose fed-batch (1 h, 15 h, 27 h, 41 h, 53 h and 67 h after starting the glucose fed-batch) referred to the glycerol fed-batch (log2 fold change). Figure S7. Principal component analysis (variable factor map; F3 vs. F2) of the glucose fed-batch (1 h, 15 h, 27 h, 41 h, 53 h and 67 h after starting the glucose fed-batch) referred to the glycerol fed-batch (log2 fold change)