Effects of Allium sativum and Allium cepa on semen characteristics, sperm reserves and haematology of rabbit bucks

The effect of dietary inclusion of garlic and onion on semen characteristics, gonadal, extragonadal sperm reserves and haematology of rabbit bucks were evaluated. Twenty-four rabbit bucks of average age and weight 10 ± 2.0 months and 1.47 ± 0.01 kg respectively, were used for the study. They were randomly assigned into four groups of six bucks each. Group A served as control, while Groups B, C and D received dietary inclusion of 5% garlic, 5% onion, 2.5% garlic + 2.5% onion, respectively. The fresh bulbs of garlic and onions were peeled, air-dried, and the dried bulbs were weighed, added to the feed and grounded together to form experimental diets. Before dietary supplementation, semen samples were collected to serve as baseline values, followed by weekly collections for another 9 weeks using an artificial vagina. Haematological parameters were examined according to the standard procedure, while testosterone profile was conducted using the ELISA method. At the termination of the experiment, two bucks from each group were euthanised, and the testes were harvested to evaluate gonadal and extragonadal sperm reserves. No significant (p > 0.05) difference was recorded in the live weight, sperm concentration, and sperm abnormality of the rabbit bucks. A significant (p < 0.05) difference was observed in the ejaculate volume, gross motility, pH, reaction time, and percentage of live spermatozoa. The epididymal sperm reserves in group B, was significantly (p < 0.05) higher in the right than left. Testosterone profile showed significant (p < 0.05) difference at 9 and 10 a.m. On haematology, there was a significant increase in PCV, RBC count, haemoglobin concentration and WBC count in groups B, C and D by week 9 compared to the control. In conclusion, the dietary inclusion of garlic and onion effectively improved the spermiogram of rabbit bucks

Anti-diarrhoeal effects of Garcinia kola (Heckel-Holl) seed methanolic extract and its fractions in animal models

This study investigated the In vitro spasmolytic and In vivo anti-diarrhoeal effects of Garcinia kola seed extract/fractions. Extraction was done by maceration in 70% methanol, serially partitioned in ethyl acetate and n-hexane. Qualitative phytochemical screening was carried out on the crude extract/fractions. The In vitro spasmolytic effect of the extract and fractions at different concentrations (0.5×103, 0.2×103,0.1×103, 0.6×102 mg/ml) were investigated against spontaneous and acetylcholine-induced contractions in isolated rabbit jejunum as well as histamine-induced contractions using isolated guinea pig ileum. The In vivo anti-diarrhoeal effect of the extract was evaluated using three diarrhoeal models: castor oil-induced diarrhoea, charcoal meal gastrointestinal transit time and castor oil-induced enteropooling. In each model, 25 mice were randomly divided into five groups of 5 mice each. Group I served as the untreated control, while group II was a positive control. Groups III-V were administered 125, 250 and 500 mg/kg of the crude methanol extract, respectively. The crude extract, ethyl acetate and aqueous fractions at 0.5×103 mg/ml respectively exhibited 14.4%, 12.9% and 12.2% spasmolytic activities against acetylcholine-induced rabbit jejunum contractions. Histamine-induced guinea pig ileum contractions were inhibited by crude extract (6.2%), ethyl acetate (6.2%), aqueous fraction (7.2%) at 0.6×102 mg/ml. For castor oil-induced diarrhoea, the crude extract at 500 mg/kg produced a significant (p < 0.05) decrease in the diarrhoeal index and faecal weight with a percentage inhibition of 70.4% compared with controls. Similarly, the crude extract (500 mg/kg) significantly (p < 0.05) decreased the charcoal meal gastrointestinal transit time with a percentage inhibition of 33.9% and elicited significant (p < 0.05) intraluminal fluid reduction (9.1%) in castor oil-induced enter pooling test when compared with the untreated group. In conclusion, the anti-motility and anti-secretory activities of the crude extract were attributed to the phytochemical constituents present

In vitro and in vivo changes observed in Trypanosoma brucei brucei-infected rats treated with artesunate and/or diminazene aceturate

This study evaluated in vitro and in vivo antitrypanosomal effect of artesunate and/or diminazene aceturate in Wistar rats experimentally  infected with Trypanosoma brucei brucei. In vitro screening was carried out in triplicates using 50 μl of 0.2, 2 and 20 μg/μl of artesunate as test drug; diminazene aceturate, normal saline and trypanosome-infected blood served as controls in a 96-well microtitre plate, incubated at 37˚C for 5 minutes. Efficacy was observed over a period of 60 minutes for reduced or complete trypanosomal immobilization. Results showed concentration-dependent cessation of trypanosomal motility was significantly (p < 0.001) induced by artesunate when compared to the controls. Seventy Wistar rats of both sexes weighing between 190 and 210 g were randomly divided into 7 groups (5 males and 5 females) are used for in vivo study. Groups I and II served as normal control and model control respectively. Groups III to VII were infected with Trypanosoma brucei brucei (106 trypanosomes/ml) intraperitoneally. At peak parasitaemia (8 days post-infection), group III was treated with diminazene aceturate (3.5 mg/kg) intramuscularly once while groups IV, V, VI were treated with artesunate (200, 100, 50) mg/kg orally for 5 consecutive days and group VII was treated with combination of artesunate (50 mg/kg) orally and diminazene aceturate (1.75 mg/kg) intramuscularly for 5 days. Results indicated pre-patent period of 4 days and increase in levels of parasitaemia post-inoculation. PCV, Hb concentration, RBC count, MCV, MCHC and total leucocyte count decreased significantly (p < 0.05) between days 0and 8 in groups II to VII. Following treatment, significant increases (p < 0.05) were recorded except for groups II, IV, V and VI where the rats died. Thus, combination of artesunate (50 mg/kg) and half the standard dose of diminazene aceturate was able to reduce parasitaemia and ameliorate the anaemia elicited by the trypanosomes. Keywords: Artesunate, Diminazene Aceturate, Haematology, Trypanosoma brucei brucei, Wistar rat