Behavior of the different proteins of the cadherin–catenin complex upon SIP1 induction

<p><b>Copyright information:</b></p><p>Taken from "SIP1/ZEB2 induces EMT by repressing genes of different epithelial cell–cell junctions"</p><p>Nucleic Acids Research 2005;33(20):6566-6578.</p><p>Published online 24 Nov 2005</p><p>PMCID:PMC1298926.</p><p>© The Author 2005. Published by Oxford University Press. All rights reserved</p> () Immunofluorescence microscopy of non-induced and induced DLD1Tr21/WTSIP1 cells using antibodies specific for adherens junction components. E-cadherin as well as αE-catenin, p120ctn and β-catenin became nearly undetectable at cell–cell contacts in the SIP1-induced cells. () Western blot analysis of the non-induced and induced DLD1Tr21/WTSIP1 cell line. E-cadherin and αE-catenin were downregulated at the protein level in the SIP1-expressing cells. β-catenin protein levels were unaltered in the SIP1-induced compared to non-induced cells. Protein expression of p120ctn isoform 1 was upregulated and that of isoform 3 downregulated after SIP1 induction (: addition of Dox every 2 days, : washing away Dox from the cell culture medium)