3,420 research outputs found
A low-voltage activated, transient calcium current is responsible for the time-dependent depolarizing inward rectification of rat neocortical neurons in vitro
Intracellular recordings were obtained from rat neocortical neurons in vitro. The current-voltage-relationship of the neuronal membrane was investigated using current- and single-electrode-voltage-clamp techniques. Within the potential range up to 25 mV positive to the resting membrane potential (RMP: –75 to –80 mV) the steady state slope resistance increased with depolarization (i.e. steady state inward rectification in depolarizing direction). Replacement of extracellular NaCl with an equimolar amount of choline chloride resulted in the conversion of the steady state inward rectification to an outward rectification, suggesting the presence of a voltage-dependent, persistent sodium current which generated the steady state inward rectification of these neurons. Intracellularly injected outward current pulses with just subthreshold intensities elicited a transient depolarizing potential which invariably triggered the first action potential upon an increase in current strength. Single-electrode-voltage-clamp measurements reveled that this depolarizing potential was produced by a transient calcium current activated at membrane potentials 15–20 mV positive to the RMP and that this current was responsible for the time-dependent increase in the magnitude of the inward rectification in depolarizing direction in rat neocortical neurons. It may be that, together with the persistent sodium current, this calcium current regulates the excitability of these neurons via the adjustment of the action potential threshold
SDSS J092455.87+021924.9: an Interesting Gravitationally Lensed Quasar from the Sloan Digital Sky Survey
We report the discovery of a new gravitationally lensed quasar from the Sloan
Digital Sky Survey, SDSS J092455.87+021924.9 (SDSS J0924+0219). This object was
selected from among known SDSS quasars by an algorithm that was designed to
select another known SDSS lensed quasar (SDSS 1226-0006A,B). Five separate
components, three of which are unresolved, are identified in photometric
follow-up observations obtained with the Magellan Consortium's 6.5m Walter
Baade telescope at Las Campanas Observatory. Two of the unresolved components
(designated A and B) are confirmed to be quasars with z=1.524; the velocity
difference is less than 100 km sec^{-1} according to spectra taken with the W.
M. Keck Observatory's Keck II telescope on Mauna Kea. A third stellar
component, designated C, has the colors of a quasar with redshift similar to
components A and B. The maximum separation of the point sources is 1.78". The
other two sources, designated G and D, are resolved. Component G appears to be
the best candidate for the lensing galaxy. Although component D is near the
expected position of the fourth lensed component in a four image lens system,
its properties are not consistent with being the image of a quasar at z~1.5.
Nevertheless, the identical redshifts of components A and B and the presence of
component C strongly suggest that this object is a gravitational lens. Our
observations support the idea that a foreground object reddens the fourth
lensed component and that another unmodeled effect (such as micro- or
milli-lensing) demagnificates it, but we cannot rule out the possibility that
SDSS0924+0219 is an example of the relatively rare class of ``three component''
lens systems.Comment: 24 pages, 6 figures, accepted by A
An Improved Photometric Calibration of the Sloan Digital Sky Survey Imaging Data
We present an algorithm to photometrically calibrate wide field optical
imaging surveys, that simultaneously solves for the calibration parameters and
relative stellar fluxes using overlapping observations. The algorithm decouples
the problem of "relative" calibrations, from that of "absolute" calibrations;
the absolute calibration is reduced to determining a few numbers for the entire
survey. We pay special attention to the spatial structure of the calibration
errors, allowing one to isolate particular error modes in downstream analyses.
Applying this to the Sloan Digital Sky Survey imaging data, we achieve ~1%
relative calibration errors across 8500 sq.deg. in griz; the errors are ~2% for
the u band. These errors are dominated by unmodelled atmospheric variations at
Apache Point Observatory. These calibrations, dubbed "ubercalibration", are now
public with SDSS Data Release 6, and will be a part of subsequent SDSS data
releases.Comment: 16 pages, 17 figures, matches version accepted in ApJ. These
calibrations are available at http://www.sdss.org/dr
Quantum Monte Carlo in the Interaction Representation --- Application to a Spin-Peierls Model
A quantum Monte Carlo algorithm is constructed starting from the standard
perturbation expansion in the interaction representation. The resulting
configuration space is strongly related to that of the Stochastic Series
Expansion (SSE) method, which is based on a direct power series expansion of
exp(-beta*H). Sampling procedures previously developed for the SSE method can
therefore be used also in the interaction representation formulation. The new
method is first tested on the S=1/2 Heisenberg chain. Then, as an application
to a model of great current interest, a Heisenberg chain including phonon
degrees of freedom is studied. Einstein phonons are coupled to the spins via a
linear modulation of the nearest-neighbor exchange. The simulation algorithm is
implemented in the phonon occupation number basis, without Hilbert space
truncations, and is exact. Results are presented for the magnetic properties of
the system in a wide temperature regime, including the T-->0 limit where the
chain undergoes a spin-Peierls transition. Some aspects of the phonon dynamics
are also discussed. The results suggest that the effects of dynamic phonons in
spin-Peierls compounds such as GeCuO3 and NaV2O5 must be included in order to
obtain a correct quantitative description of their magnetic properties, both
above and below the dimerization temperature.Comment: 23 pages, Revtex, 11 PostScript figure
The Human Fungal Pathogen Cryptococcus neoformans Escapes Macrophages by a Phagosome Emptying Mechanism That Is Inhibited by Arp2/3 Complex-Mediated Actin Polymerisation
The lysis of infected cells by disease-causing microorganisms is an efficient but risky strategy for disseminated infection, as it exposes the pathogen to the full repertoire of the host's immune system. Cryptococcus neoformans is a widespread fungal pathogen that causes a fatal meningitis in HIV and other immunocompromised patients. Following intracellular growth, cryptococci are able to escape their host cells by a non-lytic expulsive mechanism that may contribute to the invasion of the central nervous system. Non-lytic escape is also exhibited by some bacterial pathogens and is likely to facilitate long-term avoidance of the host immune system during latency. Here we show that phagosomes containing intracellular cryptococci undergo repeated cycles of actin polymerisation. These actin ‘flashes’ occur in both murine and human macrophages and are dependent on classical WASP-Arp2/3 complex mediated actin filament nucleation. Three dimensional confocal imaging time lapse revealed that such flashes are highly dynamic actin cages that form around the phagosome. Using fluorescent dextran as a phagosome membrane integrity probe, we find that the non-lytic expulsion of Cryptococcus occurs through fusion of the phagosome and plasma membranes and that, prior to expulsion, 95% of phagosomes become permeabilised, an event that is immediately followed by an actin flash. By using pharmacological agents to modulate both actin dynamics and upstream signalling events, we show that flash occurrence is inversely related to cryptococcal expulsion, suggesting that flashes may act to temporarily inhibit expulsion from infected phagocytes. In conclusion, our data reveal the existence of a novel actin-dependent process on phagosomes containing cryptococci that acts as a potential block to expulsion of Cryptococcus and may have significant implications for the dissemination of, and CNS invasion by, this organism.\ud
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The Interstellar Environment of our Galaxy
We review the current knowledge and understanding of the interstellar medium
of our galaxy. We first present each of the three basic constituents - ordinary
matter, cosmic rays, and magnetic fields - of the interstellar medium, laying
emphasis on their physical and chemical properties inferred from a broad range
of observations. We then position the different interstellar constituents, both
with respect to each other and with respect to stars, within the general
galactic ecosystem.Comment: 39 pages, 12 figures (including 3 figures in 2 parts
Phase transitions in biological membranes
Native membranes of biological cells display melting transitions of their
lipids at a temperature of 10-20 degrees below body temperature. Such
transitions can be observed in various bacterial cells, in nerves, in cancer
cells, but also in lung surfactant. It seems as if the presence of transitions
slightly below physiological temperature is a generic property of most cells.
They are important because they influence many physical properties of the
membranes. At the transition temperature, membranes display a larger
permeability that is accompanied by ion-channel-like phenomena even in the
complete absence of proteins. Membranes are softer, which implies that
phenomena such as endocytosis and exocytosis are facilitated. Mechanical signal
propagation phenomena related to nerve pulses are strongly enhanced. The
position of transitions can be affected by changes in temperature, pressure, pH
and salt concentration or by the presence of anesthetics. Thus, even at
physiological temperature, these transitions are of relevance. There position
and thereby the physical properties of the membrane can be controlled by
changes in the intensive thermodynamic variables. Here, we review some of the
experimental findings and the thermodynamics that describes the control of the
membrane function.Comment: 23 pages, 15 figure
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