Protein import into the endosymbiotic organelles of apicomplexan parasites

The organelles of endosymbiotic origin, plastids, and mitochondria, evolved through the serial acquisition of endosymbionts by a host cell. These events were accompanied by gene transfer from the symbionts to the host, resulting in most of the organellar proteins being encoded in the cell nuclear genome and trafficked into the organelle via a series of translocation complexes. Much of what is known about organelle protein translocation mechanisms is based on studies performed in common model organisms; e.g., yeast and humans or Arabidopsis. However, studies performed in divergent organisms are gradually accumulating. These studies provide insights into universally conserved traits, while discovering traits that are specific to organisms or clades. Apicomplexan parasites feature two organelles of endosymbiotic origin: a secondary plastid named the apicoplast and a mitochondrion. In the context of the diseases caused by apicomplexan parasites, the essential roles and divergent features of both organelles make them prime targets for drug discovery. This potential and the amenability of the apicomplexan Toxoplasma gondii to genetic manipulation motivated research about the mechanisms controlling both organelles’ biogenesis. Here we provide an overview of what is known about apicomplexan organelle protein import. We focus on work done mainly in T. gondii and provide a comparison to model organisms

Perturbative correction to the adiabatic approximation for (d,p)(d,p) reactions

The adiabatic distorted wave approximation (ADWA) is widely used by the nuclear community to analyse deuteron stripping ($d$,$p$) experiments. It provides a quick way to take into account an important property of the reaction mechanism: deuteron breakup. In this work we provide a numerical quantification of a perturbative correction to this theory, recently proposed in [R.C. Johnson, J. Phys. G: Nucl. Part. Phys. 41 (2014) 094005] for separable rank-one nucleon-proton potentials. The correction involves an additional, nonlocal, term in the effective deuteron-target ADWA potential in the entrance channel. We test the calculations with perturbative corrections against continuum-discretized coupled channel predictions which treat deuteron breakup exactly

Lean Daily Management: Keeping Quality and Safety at the Forefront During the COVID-19 Pandemic

This storyboard describes how our Operational Excellence Platform pivoted to keep quality at the forefront of the Covid-19 response. Applying the Framework for Safe Effective and Reliable Care, we implemented the following innovations: Daily quality huddles became virtual, with 70-100 frontline leaders sharing alerts, announcements and celebrations Traditional Gemba Walks transformed into daily Executive Gemba Rounds visiting all care teams and focusing on patient and workforce safety. Patient and Workforce safety concerns were resolved real time and those requiring coordinated response were triaged to the Hospital Incident Command System (HICS) for accelerated action. Innovative communication strategies were adopted to clarify changing practice in midst of the pandemic, to message adherence to personal protective equipment standards, and improved operational workflows for efficient problem solving

Protein palmitoylation plays an important role in Trichomonas vaginalis adherence

The flagellated protozoan parasite Trichomonas vaginalis is the etiologic agent of trichomoniasis, the most common non-viral sexually transmitted infection worldwide. As an obligate extracellular pathogen, adherence to epithelial cells is critical for parasite survival within the human host and a better understanding of this process is a prerequisite for the development of therapies to combat infection. In this sense, recent work has shown S-acylation as a key modification that regulates pathogenesis in different protozoan parasites. However, there are no reports indicating whether this post-translational modification is a mechanism operating in T. vaginalis. In order to study the extent and function of S-acylation in T. vaginalis biology, we undertook a proteomic study to profile the full scope of S-acylated proteins in this parasite and reported the identification of 363 proteins involved in a variety of biological processes such as protein transport, pathogenesis related and signaling, among others. Importantly, treatment of parasites with the palmitoylation inhibitor 2-bromopalmitate causes a significant decrease in parasite: Parasite aggregation as well as adherence to host cells suggesting that palmitoylation could be modifying proteins that are key regulators of Trichomonas vaginalis pathogenesis.Fil: Nievas, Yésica Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaFil: Vashisht, Ajay A.. University of California; Estados UnidosFil: Corvi, Maria Martha. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaFil: Metz, Sebastián Darío. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaFil: Johnson, Patricia J. University of California; Estados UnidosFil: Wohlschlegel, James A.. University of California; Estados UnidosFil: de Miguel, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentin

Alternative Building Materials (ABM): Towards Adoption of Common Terminology and Definitions

There are differences in the definitions of Alternative Building Materials (ABM) and differences in the terminologies that are used in describing ABM by different researchers in the construction industry. ABM is a generic term, which is characterised of or relating to a class or group of building materials, not really specific. It encompasses building and construction materials that in literature are referred to by different names such as alternative materials, local building materials, unconventional building materials, alternative residential construction materials, sustainable building materials, indigenous building materials, vernacular building materials, green building materials, environmentally responsible building materials, eco-friendly building materials, etc. The research employed systematic literature review and content analysis to generate and analysedall the necessary information as the methodology.  A working (operational) definition of Alternative Building Materials is being offered as building materials that are an alternative to conventional building materials in the form of total or partial substitution of the materials or its constituents for the purpose of reducing the cost, addressing environmental issues or dealing with lack of conventional materials. The characteristics of ABM have been identified to include low or no chemical emissions that can lead to poor indoor air quality, recycled content (post-consumer and pre-consumer), no CFC, HCFC, or other ozone depleting substances content, low embodied energy, locally produced, possibility of repairs and replacements with local means and social acceptability amongst others. Some of the benefits of utilizing ABM include; low embodied energy (often leading to reduced greenhouse gas emissions), ease of construction, widespread availability and low cost. Keywords: Alternative Building Materials, Definition, Adoption, Terminology, Characteristics DOI: 10.7176/CER/12-11-03 Publication date: November 30th 202