Shifting Patterns of Nitrogen Excretion and Amino Acid Catabolism Capacity during the Life Cycle of the Sea Lamprey (\u3cem\u3ePetromyzon mariunus\u3c/em\u3e)

The jawless fish, the sea lamprey (Petromyzon marinus), spends part of its life as a burrow-dwelling, suspension-feeding larva (ammocoete) before undergoing a metamorphosis into a free swimming, parasitic juvenile that feeds on the blood of fishes. We predicted that animals in this juvenile, parasitic stage have a great capacity for catabolizing amino acids when large quantities of protein-rich blood are ingested. The sixfold to 20-fold greater ammonia excretion rates (JAmm) in postmetamorphic (nonfeeding) and parasitic lampreys compared with ammocoetes suggested that basal rates of amino acid catabolism increased following metamorphosis. This was likely due to a greater basal amino acid catabolizing capacity in which there was a sixfold higher hepatic glutamate dehydrogenase (GDH) activity in parasitic lampreys compared with ammocoetes. Immunoblotting also revealed that GDH quantity was 10-fold and threefold greater in parasitic lampreys than in ammocoetes and upstream migrant lampreys, respectively. Higher hepatic alanine and aspartate aminotransferase activities in the parasitic lampreys also suggested an enhanced amino acid catabolizing capacity in this life stage. In contrast to parasitic lampreys, the twofold larger free amino acid pool in the muscle of upstream migrant lampreys confirmed that this period of natural starvation is accompanied by a prominent proteolysis. Carbamoyl phosphate synthetase III was detected at low levels in the liver of parasitic and upstream migrant lampreys, but there was no evidence of extrahepatic (muscle, intestine) urea production via the ornithine urea cycle. However, detection of arginase activity and high concentrations of arginine in the liver at all life stages examined infers that arginine hydrolysis is an important source of urea. We conclude that metamorphosis is accompanied by a metabolic reorganization that increases the capacity of parasitic sea lampreys to catabolize intermittently large amino acid loads arising from the ingestion of protein rich blood from their prey/hosts. The subsequent generation of energy-rich carbon skeletons can then be oxidized or retained for glycogen and fatty acid synthesis, which are essential fuels for the upstream migratory and spawning phases of the sea lamprey’s life cycle

Historical and Projected Distributions of Daily Temperature and Pressure in the Arctic

Changes in extreme temperatures and pressures in the Arctic have received little attention in the context of climate change. Here we examine the distributions and extremes of surface air temperature and pressure in the Arctic for the late 20th century, using Alaskan weather station data, an atmospheric reanalysis, and general circulation models (GCMs). There is good agreement among these sources for the late 20th century, with broader distributions for both temperature and pressure in winter as compared to summer, and over land as compared to over ocean. We used the output from 21st-century greenhouse simulations by the GCMs to address the occurrence of extremes in the coming decades. The model projections of the 21st-century extremes largely agree with changes in the mean state, with record low temperatures decreasing in frequency and record high temperatures increasing in frequency. The changes in 21st-century extremes are more pronounced over the ocean, where the present-day distributions are narrower. The projected decreases of mean pressure result in more frequent occurrences of extreme low pressure, especially over the Arctic Ocean, although the extremes of pressure are less affected by changes of the means than are the extremes of temperature. Lastly, we find that the transition from sea ice to open water, and associated changes in the salinity of the surface water, can cause changes in the temperature distribution that are more complex than simple shifts in the distribution, leading to unexpected changes in the occurrence of extreme temperatures.Les changements en matière de températures et de pressions extrêmes dans l’Arctique reçoivent peu d’attention dans le contexte du changement climatique. Ici, nous examinons les répartitions et les extrêmes de la température de l’air en surface et de la pression dans l’Arctique vers la fin du XXe siècle et ce, en nous appuyant sur les données de la station météorologique de l’Alaska, sur une nouvelle analyse atmosphérique et sur des modèles de circulation générale. Il existe une bonne concordance quant aux sources visant la fin du XXe siècle, avec des répartitions plus vastes pour les températures et pressions d’hiver comparativement à celles de l’été, ainsi que pour les températures et pressions terrestres par rapport aux températures et pressions océaniques. Nous nous sommes également servi des données émanant de simulations de serres au XXIe siècle, réalisées au moyen des modèles de circulation générale afin d’être en mesure de nous pencher sur l’occurrence des extrêmes au cours des décennies à venir. Les projections de modèles relatives aux extrêmes du XXIe siècle concordent largement avec les changements caractérisant l’état de la moyenne, la fréquence des basses températures record étant à la baisse et la fréquence des hautes températures record étant à la hausse. Les changements en matière d’extrêmes au XXIe siècle sont plus prononcés au-dessus de l’océan, là où les répartitions actuelles sont plus étroites. Les diminutions projetées de la pression moyenne se traduisent par des occurrences plus fréquentes de basses pressions extrêmes, surtout au-dessus de l’océan Arctique, bien que les extrêmes en matière de pressions soient moins touchés par les changements des moyennes que les extrêmes de températures. De plus, nous estimons que la transition allant de la glace de mer à l’eau libre, de même que les changements connexes du point de vue de la salinité de l’eau de surface, peuvent entraîner des changements sur le plan de la répartition des températures qui sont plus complexes que de simples décalages de répartition, ce qui mène à des changements inattendus en matière d’occurrence de températures extrêmes

Effects of Amphetamine on Striatal Dopamine Release, Open-Field Activity, and Play in Fischer 344 and Sprague–Dawley Rats

Previous work from our laboratories has shown that juvenile Fischer 344 (F344) rats are less playful than other strains and also appear to be compromised in dopamine (DA) functioning. To determine whether the dysfunctional play in this strain is associated with deficits in the handling and delivery of vesicular DA, the following experiments assessed the extent to which F344 rats are differentially sensitive to the effects of amphetamine. When exposed to amphetamine, striatal slices obtained from F344 rats showed a small increase in unstimulated DA release when compared with slices from Sprague–Dawley rats; they also showed a more rapid high K+-mediated release of DA. These data provide tentative support for the hypothesis that F344 rats have a higher concentration of cytoplasmic DA than Sprague–Dawley rats. When rats were tested for activity in an open field, F344 rats presented a pattern of results that was consistent with either an enhanced response to amphetamine (3 mg/kg) or a more rapid release of DA (10 mg/kg). Although there was some indication that amphetamine had a dose-dependent differential effect on play in the two strains, play in F344 rats was not enhanced to any degree by amphetamine. Although these results are not consistent with our working hypothesis that F344 rats are less playful because of a deficit in vesicular release of DA, they still suggest that this strain may be a useful model for better understanding the role of DA in social behavior during the juvenile period

An analysis of factors affecting oxygen depletion in the New York Bight

Low oxygen water, of varying spatial extent, has been observed during the summer over past years in the New York Bight. In the summer of 1976 a $60 million loss of shellfish resulted from anoxia along the New Jersey coast. The development of anoxia has been attributed to increased anthropogenic carbon loading from urban areas adjacent to the Bight..

An energy efficient power controller switching methodology for an ambient healthcare network

A methodology for improved power controller switching in mobile Body Area Networks operating within the ambient healthcare environment is proposed. The work extends Anti-windup and Bumpless transfer results to provide a solution to the ambulatory networking problem that ensures sufficient biometric data can always be regenerated at the base station. The solution thereby guarantees satisfactory quality of service for healthcare providers. Compensation is provided for the nonlinear hardware constraints that are a typical feature of the type of network under consideration and graceful performance degradation in the face of hardware output power saturation is demonstrated, thus conserving network energy in an optimal fashion

A medical study on wireless inertial measurement technology as a tool for identifying patients at risk of death or imminent clinical deterioration

This paper provides a system description and preliminary results for an ongoing clinical study currently being carried out at the Mid-Western Regional Hospital, Nenagh, Ireland. The goal of the trial is to determine if wireless inertial measurement technology can be employed to identify elderly patients at risk of death or imminent clinical deterioration. The system measures cumulative movement and provides a score that will help provide a robust early warning to clinical staff of clinical deterioration. In addition the study examines some of the logistical barriers to the adoption of wearable wireless technology in front-line medical care

Capturing the overarm throw in darts employing wireless inertial measurement

This work employs a custom built body area network of wireless inertial measurement technology to conduct a biomechanical analysis of precision targeted throwing in competitive and recreational darts. The solution is shown to be capable of measuring key biomechanical factors including speed, acceleration and timing. These parameters are subsequently correlated with scoring performance to determine the affect each variable has on outcome. For validation purposes an optical 3D motion capture system provides a complete kinematic model of the subject and enables concurrent benchmarking of the 'gold standard' optical inertial measurement system with the more affordable and proactive wireless inertial measurement solution developed as part of this work

A multi-technology approach to identifying the reasons for lateral drift in professional and recreational darts

This work performs an extensive charterisation of precision targeted throwing in professional and recreational darts. The goal is to identify the contributing factors for lateral drift or throwing inaccuracy in the horizontal plane. A multitechnology approach is adopted whereby a custom built body area network of wireless inertial measurement devices monitor tilt, force and timing, an optical 3D motion capture system provides a complete kinematic model of the subject, electromyography sensors monitor muscle activation patterns and a force plate and pressure mat capture tactile pressure and force measurements. The study introduces the concept of constant throwing rhythm and highlights how landing errors in the horizontal plane can be attributable to a number of variations in arm force and speed, centre of gravity and the movements of some of the bodies non throw related extremities

A preliminary study on how fatigue affects scoring average in professional darts

The purpose of this preliminary study is to identify signs of fatigue in specific muscle groups that in turn directly influence accuracy in professional darts. Electromyography (EMG) sensors are employed to monitor the electrical activity produced by skeletal muscles of the trunk and upper limb during throw. It is noted that the Flexor Pollicis Brevis muscle which controls the critical release action during throw shows signs of fatigue. This is accompanied by an inherent increase in mean integral EMG amplitude for a number of other throw related muscles indicating an attempt to maintain constant applied throwing force. A strong correlation is shown to exist between average score and decrease in mean integral ECG amplitude for the Flexor Pollicis Brevis

A benchmark comparison between reconfigurable, intelligent and autonomous wireless inertial measurement and photonic technologies in rehabilitation

Advanced sensory systems address a number of major obstacles towards the provision for cost effective and proactive rehabilitation. Many of these systems employ technologies such as high-speed video or motion capture to generate quantitative measurements. However these solutions are accompanied by some major limitations including extensive set-up and calibration, restriction to indoor use, high cost and time consuming data analysis. Additionally many do not quantify improvement in a rigorous manner for example gait analysis for 5 minutes as opposed to 24 hour ambulatory monitoring. This work addresses these limitations using low cost, wearable wireless inertial measurement as a mobile and minimal infrastructure alternative. In cooperation with healthcare professionals the goal is to design and implement a reconfigurable and intelligent movement capture system. A key component of this work is an extensive benchmark comparison with the 'gold standard' VICON motion capture system