Intracellular F-actin reorganization by is dependent on the Lys of SteC

<p><b>Copyright information:</b></p><p>Taken from "SteC is a kinase required for SPI-2-dependent F-actin remodelling"</p><p></p><p>Cellular Microbiology 2008;10(1):20-30.</p><p>Published online Jan 2008</p><p>PMCID:PMC2253691.</p><p>© 2007 The Authors Journal compilation © 2007 Blackwell Publishing Ltd</p> A. Representative confocal images of Swiss 3T3 fibroblasts infected for 8 h with an mutant containing plasmid-borne , or . Cells were immunolabelled to detect (green in merged image). F-actin was visualized by phalloidin-RRX staining (red in merged image). Bars represent 5 μm. B. Quantification of F-actin reorganization by strains. Results are the means ± SE of three independent experiments, in which a total of 300 infected cells were examined for each strain. Results for cells infected with wild-type or mutant strains are for comparison and are from . C. Representative confocal images of infected cells showing translocation of SteC-2HA and SteCK256H-2HA expressed from a low-copy plasmid. Cells were fixed and immunolabelled to detect (green in merged image) and HA (red in merged image). Bars represent 5 μm. D. Representative confocal micrograph of an infected cell showing translocated SteC-2HA localizing to F-actin. Cells were fixed and immunolabelled to detect (blue in merged image) and HA (green in merged image. F-actin was visualized by phalloidin-RRX staining (red in merged image). Boxed insets are magnifications of indicated region showing localization of SteC-2HA with F-actin. Bars represent 5 μm