Synthesis and properties of selected alpha amylase inhibitors

A logical approach to the design of [alpha]-amylase inhibitors is developed from the known features of catalysis by glycosidases in general and [alpha]-amylases in particular. Four potential inhibitors of wheat a-amylase namely maltobiono-cf-lactone, maltal, N-D-glucosylbenzyl-amine and N-D-glucosylpiperidine have been synthesised and their structure established by a variety of physical methods. 1H NMR has shown maltal to exist either as an equilibrium system involving the and conformations, with the predominating, or possibly as a single conformation slightly distorted from the classical half chair. Both N-D-glucosylbenzylamine and N-D-glucosylpiperidine were shown to exist in the 3-form with the normal chair conformation of the sugar ring. The substituted piperidine ring undergoes rapid ring inversion at ambient temperature. For the inhibition studies, wheat g-amylase was extracted from a sample of malted Champlein wheat and obtained with a specific activity of 44 Units per mg of protein. Inhibition by maltobiono-s-lactone and maltal was examined at pH 5.0 by analysing for reducing sugar production using the alkaline ferricyanide method, Maltoiono-c-lactone was shown to be a non-competitive inhibitor with an inhibition constant of 2.5 mM. Maltal behaved as a slow-binding inhibitor, and atequilibrium (which was reached after about one hour undertest conditions) acted competitively with an inhibition constant of 4.4 mM. Inhibition by the N-glycosylamines was complicated by their significant hydrolysis at pH 5, and was examined at pH 6 using an iodine stain technique. N-D-glucosylbenylamine and N-D-glucosyl-t piperidine were moderate inhibitors of wheat a-amylase. Their inhibition behaviour was complex (non-linear), possibly because of concomitant hydrolysis.<p

The Ursinus Weekly, February 25, 1963

1962 Messiah recording delivered to White House by UC delegation • Young Republicans hear Staudinger on reapportionment • Changes sought in WSGA rules • Mayer tells audience communism\u27s faults • G. Dolman to speak at Methacton High seminar • Sears presents grant of $750 to Ursinus • 1962 Campus Chest begins next week • Half of students get financial aid • Dr. Creager author of 3 religious works • Sororities and fraternities pledge new members as Spring rushing ends • Spring Festival planning underway; Grace Folwell elected queen of court • Staiger gives lecture at Villanova seminar • Only 35 students donate blood • Drew\u27s Michalson gives lecture on existentialism • New parking regulations instituted in town • Staiger\u27s Disney-type films shown at Beardwood Chem meeting • Film offerings of high quality • Editorial: Those letters to the editor • Dr. F tosses pearls to swine • Greek gleanings • Error revealed in Weekly claim of publication year • Review of the Lantern by Gustavson\u27s 2 selves • Cagers edge Drew 66-64 for 3rd win, lose to Drexel 69-53 on Saturday • Matmen dump Lebanon Valley, suffer upset at PMC on Saturday • Track squad members join practice meet • Siebenson shows improvement • Mermaids splash to 3 victories • Netwomen defeat Wilson, Rosemonthttps://digitalcommons.ursinus.edu/weekly/1288/thumbnail.jp

The Ursinus Weekly, March 23, 1964

Campus Chest activities will begin April 6th • Craig Hill named Weekly editor-in-chief; Bell, Bradley and Davis will head staffs • Nelson Bortz April 8 Forum guest speaker: Labor - management relations topic • Peek to study in Munich • Miller featured in Cuban program: TV appearance set on Great Decisions, 1964 • Students and faculty enter benefit show: Talent and auction for Campus Chest • Spring Festival chairmen reveal plans for musical • Informal rush planned in April: Sorority parties • APO seeks funds for amplification system in gym • Students organize to fight against discrimination • Class of \u2767 sponsors coffee house dance • Editorial: Whatever we\u27ve done • Letters to the editor • Should students 21 be restricted from legal privileges: Student drinking • Forum: Dr. John Noss; Inter-religious communication subject of talk • New dining hall • Is sex education a moral issue? • IRC participates in thirty-seventh National Model General Assembly • Greek gleanings • Dr. J.E. Wagner guest preacher for Holy Week • Kitty Award • Dean\u27s list • Women cagers fall in overtime to West Chester • Mermaids sweep Swarthmore, 48-21 • Shreiner unbeaten: Playoff Tuesday • Intramural champ, Bock, challenges all star teamhttps://digitalcommons.ursinus.edu/weekly/1269/thumbnail.jp

Improved atomic data for electron-transport predictions by the codes TIGER and TIGERP. I. Inner-shell ionization by electron collision

The inner-shell ionization data for electron-target collisions now in use in the TIGER and TIGERP electron-transport codes are extracted and compared with other data for these processes. The TIGER cross sections for K-shell ionization by electron collisions are found to be seriously in error for large-Z targets and incident electron energies greater than 1 MeV. A series of TIGER and TIGERP runs were carried out with and without improved K-shell electron ionization cross section data replacing that now in use. The relative importance of electron-impact and photon ionization of the various subshells was also extracted from these runs. In general, photon ionization dominated in the examples studied so the sensitivity of many predicted properties to errors in the electron-impact subshell ionization data was not large. However, some differences were found and, as all possible applications were not covered in this study, it is recommended that these electron-impact data now in TIGER and TIGERP be replaced. Cross section data for the processes under study are reviewed and those that are most suitable for this application are identified. 19 references, 9 figures, 2 tables

Audit of the autoantibody test, EarlyCDT®-Lung, in 1600 patients: An evaluation of its performance in routine clinical practice

ObjectivesEarlyCDT®-Lung may enhance detection of early stage lung cancer by aiding physicians in assessing high-risk patients through measurement of biological markers (i.e., autoantibodies). The test's performance characteristics in routine clinical practice were evaluated by auditing clinical outcomes of 1613 US patients deemed at high risk for lung cancer by their physician, who ordered the EarlyCDT-Lung test for their patient.MethodsClinical outcomes for all 1613 patients who provided HIPAA authorization are reported. Clinical data were collected from each patient's treating physician. Pathology reports when available were reviewed for diagnostic classification. Staging was assessed on histology, otherwise on imaging.ResultsSix month follow-up for the positives/negatives was 99%/93%. Sixty-one patients (4%) were identified with lung cancer, 25 of whom tested positive by EarlyCDT-Lung (sensitivity = 41%). A positive EarlyCDT-Lung test on the current panel was associated with a 5.4-fold increase in lung cancer incidence versus a negative. Importantly, 57% (8/14) of non-small cell lung cancers detected as positive (where stage was known) were stage I or II.ConclusionsEarlyCDT-Lung has been extensively tested and validated in case–control settings and has now been shown in this audit to perform in routine clinical practice as predicted. EarlyCDT-Lung may be a complementary tool to CT for detection of early lung cancer

A New Planet Around an M Dwarf: Revealing a Correlation Between Exoplanets and Stellar Mass

We report precise Doppler measurements of GJ317 (M3.5V) that reveal the presence of a planet with a minimum mass Msini = 1.2 Mjup in an eccentric, 692.9 day orbit. GJ317 is only the third M dwarf with a Doppler-detected Jovian planet. The residuals to a single-Keplerian fit show evidence of a possible second orbital companion. The inclusion of an additional Jupiter-mass planet (P = 2700 days, Msini = 0.83 Mjup) improves the quality of fit significantly, reducing the rms from 12.5 m/s to 6.32 m/s. A false-alarm test yields a 1.1% probability that the curvature in the residuals of the single-planet fit is due to random fluctuations, lending additional credibility to the two-planet model. However, our data only marginally constrain a two-planet fit and further monitoring is necessary to fully characterize the properties of the second planet. To study the effect of stellar mass on Jovian planet occurrence we combine our samples of M stars, Solar-mass dwarfs and intermediate-mass subgiants. We find a positive correlation between stellar mass and the occurrence rate of Jovian planets within 2.5 AU; the former A-type stars in our sample are nearly 5 times more likely than the M dwarfs to harbor a giant planet. Our analysis shows that the correlation between Jovian planet occurrence and stellar mass remains even after accounting for the effects of stellar metallicity.Comment: ApJ accepted, 27 pages, 6 figures, 3 table

Compact HI clouds from the GALFA-HI survey

The Galactic Arecibo L-band Feed Array HI (GALFA-HI) survey is mapping the entire Arecibo sky at 21-cm, over a velocity range of -700 to +700 km/s (LSR), at a velocity resolution of 0.18 km/s and a spatial resolution of 3.5 arcmin. The unprecedented resolution and sensitivity of the GALFA-HI survey have resulted in the detection of numerous isolated, very compact HI clouds at low Galactic velocities, which are distinctly separated from the HI disk emission. In the limited area of ~4600 deg$^2$ surveyed so far, we have detected 96 of such compact clouds. The detected clouds are cold with a median T$_{k,max}$ (the kinetic temperature in the case in which there is no non-thermal broadening) of 300 K. Moreover, these clouds are quite compact and faint, with median values of 5 arcmin in angular size, 0.75 K in peak brightness temperature, and $5 \times 10^{18}$ cm$^{-2}$ in HI column density. Most of the clouds deviate from Galactic rotation at the 20-30 km/s level, and a significant fraction show evidence for a multiphase medium and velocity gradients. No counterparts for these clouds were found in other wavebands. From the modeling of spatial and velocity distributions of the whole compact cloud population, we find that the bulk of the compact clouds are related to the Galactic disk, and their distances are likely to be in the range of 0.1 to a few kpc. We discuss various possible scenarios for the formation and maintenance of this cloud population and its significance for Galactic ISM studies.Comment: Accepted for publication in the Astrophysical Journa

The Ursinus Weekly, February 11, 1963

President Emeritus McClure dies following recent illness: Dr. Yost pays tribute to his late colleague • Dr. Donald Baker discusses US at Koffee Klatch • Coed foursome discusses Summer with Indians • President Helfferich quizzed in Controversy at midnight chat: Topics discussed include fraternities, government aid, college isolation, library • Annual Lorelei dance scheduled for Friday evening at Sunnybrook • Legal counselor slated to address PSEA • Tennis coach displays art exhibit in Library • Living under communism topic of Wednesday\u27s Forum speaker • Bible Study film examines nature • Powers & Fuges leave UC to enlist in Peace Corps • Kachel, Berlinger elected 1964 Ruby business managers • Chi Alpha hears talk on research • Spring rushing periods begin for fraternities and sororities • Editorial: End of an era; As youth should be spent • Letters to the editor • Silver scholarships offered to coeds • Greek gleanings • Spring Mountain ski slope offers fun for both beginners and experts • Netmen downed by Haverford 80-66, eke out 65-63 win over Hopkins • Tenacious play marks Hofmann • Kratz versatile, solid, dependable • Matmen stunned by E\u27towners in 17-11 upset • Jayvee netwomen claim 46-38 win over Phila. Bible • Intramural storyhttps://digitalcommons.ursinus.edu/weekly/1286/thumbnail.jp