Additional file 1: Table S1. of Angiogenesis related genes NOS3, CD14, MMP3 and IL4R are associated to VEGF gene expression and circulating levels in healthy adults

VEGF plasma and expression levels per genotype. Table S2. VEGF plasma and expression levels per interaction condition. (DOCX 72 kb

Pro- and anti-angiogenic VEGF mRNAs in autoimmune thyroid diseases

<p>The aim of this study was to assess the relationships between five different splice isoforms of VEGF mRNA and its plasma levels in individuals treated for autoimmune thyroid diseases (AITD); mainly Graves’ disease (GD) and Hashimoto’s thyroiditis (HT). In a population from Tunisia, levels of thyroid hormones and antibodies were quantified simultaneously with plasma VEGF and VEGF mRNA isoforms after a period of 6 months of patients’ treatment. Plasma VEGF was measured in 110 AITD patients (21 GD and 89 HT patients). VEGF isoforms (VEGF121, VEGF165, VEGF145 and VEGF189 pro-angiogenic isoforms and VEGF165b anti-angiogenic isoform) in peripheral blood mononuclear cells were quantified in 71 patients (20 GD and 51 HT patients) and 86 healthy controls. Decreased levels of VEGF189 mRNA were observed in AITD compared to controls. VEGF165 was increased in GD patients compared to controls and the VEGF165b was increased in HT patients compared to GD. We observed increased levels of VEGF165b in hypothyroid AITD patients after treatment. We have also shown that the VEGF145 isoform levels were determined by FT4 in all patients and by the thyroid status after 6 months of treatment only in HT patients. An association was observed for VEGF165 mRNA levels with anti-TPO antibodies in all patients. Finally, FT4 was associated with VEGF plasma levels but only in healthy controls. In conclusion, this descriptive study highlights the specificity of VEGF mRNA isoforms in AITD, a fact underlining the need for novel clinical trials and the development of personalised theranostic approaches.</p

DataSheet1_The 10th Santorini conference: Systems medicine, personalised health and therapy. “The odyssey from hope to practice: Patient first. Keep Ithaca always in your mind”, Santorini, Greece, 23–26 May 2022.DOCX

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Meta-analysis result of the 10 replicated variants.

<p>Meta-analysis result of the 10 replicated variants.</p

Forest plots of the 10 replicated variants.

<p>Panels A-J. The effect size (Beta) and the 95% Confidence Interval (CI) in all discovery and replication studies and the cumulative effect in the inverse-variance meta-analysis are represented for each replicated variant.</p

Network generated by IPA using 26 genes in replicated loci.

<p>Edges describe the characteristics of relationship between the two nodes. Lines between two genes denote verified interaction. The shapes of the nodes represent the functional level of the gene product.</p

Flow chart for VEGF meta-analysis study design.

<p>Flow chart for VEGF meta-analysis study design.</p

Descriptive statistics for participating cohorts at all stages.

<p>Descriptive statistics for participating cohorts at all stages.</p