Periodic acid Schiff (PAS) staining.

<p>Periodic acid Schiff (PAS) stained lung sections from control (<b>A, C, E</b>) and <i>Hif-1αΔ/Δ</i> (<b>B</b>), <i>Hif-2αΔ/Δ</i> (<b>D</b>), <i>Hif-1/2αΔ/Δ</i> (<b>F</b>) pups. Cuboidal epithelium lining alveolar septa in <i>Hif-1αΔ/Δ</i> pups (<b>B</b>) has greater PAS-stained glycogen (arrows) compared to that of the control pup.</p

Pulmonary Histopathology.

<p>Light photomicrographs of hematoxylin and eosin stained lung sections from control (<b>A</b>), <i>Hif-1αΔ/Δ</i> (<b>B</b>), <i>Hif-2αΔ/Δ</i> (<b>C</b>) and <i>Hif-1/2αΔ/Δ</i> (<b>D</b>) pups. Analysis of septal thickness of control mice (<b>white bars</b>) and <i>Hif-1αΔ/Δ</i> mice (<b>black bars</b>) was measured morphometrically as described in materials and methods (<b>E</b>). Results are presented as mean alveolar thickness with error bars representing the standard error. Alveolar airspace labeled as (a), septae labeled as (S). * = P ≤ 0.05.</p

Differential gene expression in lungs of <i>Hif-1αΔ/Δ</i>, <i>Hif-2αΔ/Δ</i> and <i>Hif-1/2αΔ/Δ</i> mice.

<p>Process for identifying differentially regulated genes across all three genotypes (<b>A</b>). Comparative gene expression analysis between <i>Hif-1αΔ/Δ</i>, <i>Hif-2αΔ/Δ</i>, and <i>Hif-1/2αΔ/Δ</i> pups. The Venn diagram illustrates common and unique differentially expressed genes (<b>B</b>).</p