Multi-elemental speciation analysis of barley genotypes diering in tolerance to cadmium toxicity using SEC-ICP-MS and ESI-TOF-MS

Plants respond to Cd exposure by synthesizing heavy-metal-binding oligopeptides, called phytochelatins (PCs). These peptides reduce the activity of Cd2+ ions in the plant tissues by forming Cd chelates. The main objective of the present work was to develop an analytical technique, which allowed identication of the most prominent Cd species in plant tissue by SEC-ICP-MS and ESI-TOF-MS. An integrated part of the method development was to test the hypothesis that dierential Cd tolerance between two barley genotypes was linked to dierences in Cd speciation. Only one fraction of Cd species, ranging from 7001800 Da, was detected in the shoots of both genotypes. In the roots, two additional fractions ranging from 29004600 and 670015 000 Da were found. The Cd-rich SEC fractions were heart-cut, de-salted and demetallized using reversed-phase chromatography (RPC), followed by ESI-MS-TOF to identify the ligands. Three dierent families of PCs, viz. (gGlu-Cys)n-Gly (PCn), (gGlu-Cys)n-Ser (iso-PCn) and Cys-(gGlu-Cys)n-Gly (des-gGlu-PCn), the last lacking the N-terminal amino acid, were identied. The PCs induced by Cd toxicity also bound several essential trace elements in plants, including Zn, Cu, and Ni, whereas no Mn species were detected. Zn, Cu and Ni-species were distributed between the 7001800 Da and 670015 000 Da fractions, whereas only Cd species were found in the 29004600 Da fraction dominated by PC3 ligands. Although the total tissue concentration of Cd was similar for the two species, the tolerant barley genotype synthesized signicantly more CdPC3 species with a high Cd specicity than the intolerant genotype, clearly indicating a correlation between Cd tolerance and the CdPC speciation

Atmospheric neutron measurements with the SONTRAC science model

–The SOlar Neutron TRACking (SONTRAC) telescope was originally developed to measure the energy spectrum and incident direction of neutrons produced in solar flares, in the energy range 20 - 250 MeV. While developed primarily for solar physics, the SONTRAC detector may be employed in virtually any application requiring both energy measurement and imaging capabilities. The SONTRAC Science Model (SM) is presently being operated at the University of New Hampshire (UNH) as a ground-based instrument to investigate the energy spectrum, zenith and azimuth angle dependence of the cosmic-ray induced sea-level atmospheric neutron flux. SONTRAC measurements are based on the non-relativistic double scatter of neutrons off ambient protons within a block of scintillating fibers. Using the n-p elastic double-scatter technique, it is possible to uniquely determine the neutron’s energy and direction on an event-by-event basis. The 3D SM consists of a cube of orthogonal plastic scintillating fiber layers with 5 cm sides, read out by two CCD cameras. Two orthogonal imaging chains allow full 3D reconstruction of scattered proton tracks

Cryptococcal aortitis presenting as a ruptured mycotic abdominal aortic aneurysm

AbstractMycotic processes occasionally complicate atherosclerotic aortic disease and usually require aggressive surgical therapy to control sepsis and prevent arterial rupture. Rarely, fungal organisms are responsible for primary infection of the abdominal aorta. We report the first case of Cryptococcal aortitis presenting as a ruptured abdominal aortic aneurysm. The surgical, pathologic, and microbiologic aspects of fungal aortitis are discussed. (J Vasc Surg 1999;30:189-92.

ENSO and Variability of the Antarctic Peninsula Pelagic Marine Ecosystem

The West Antarctic Peninsula region is an important source of Antarctic krill (Euphausia superba) in the Southern Ocean. From 1980-2004 abundance and concentration of phytoplankton and zooplankton, krill reproductive and recruitment success and seasonal sea ice extent here were significantly correlated with the atmospheric Southern Oscillation Index and exhibited three- to five-year frequencies characteristic of El Nino-Southern Oscillation (ENSO) variability. This linkage was associated with movements of the Southern Antarctic Circumpolar Current Front and Boundary, a changing influence of Antarctic Circumpolar Current and Weddell Sea waters, and eastward versus westward flow and mixing processes that are consistent with forcing by the Antarctic Dipole high-latitude climate mode. Identification of hydrographic processes underlying ecosystem variability presented here were derived primarily from multidisciplinary data collected during 1990-2004, a period with relatively stable year-to-year sea ice conditions. These results differ from the overwhelming importance of seasonal sea ice development previously established using 1980-1996 data, a period marked by a major decrease in sea ice from the Antarctic Peninsula region in the late 1980s. These newer results reveal the more subtle consequences of ENSO variability on biological responses. They highlight the necessity of internally consistent long-term multidisciplinary datasets for understanding ecosystem variability and ultimately for establishing well-founded ecosystem management. Furthermore, natural environmental variability associated with interannual- and decadal-scale changes in ENSO forcing must be considered when assessing impacts of climate warming in the Antarctic Peninsula-Weddell Sea region

Density fluctuations and the structure of a nonuniform hard sphere fluid

We derive an exact equation for density changes induced by a general external field that corrects the hydrostatic approximation where the local value of the field is adsorbed into a modified chemical potential. Using linear response theory to relate density changes self-consistently in different regions of space, we arrive at an integral equation for a hard sphere fluid that is exact in the limit of a slowly varying field or at low density and reduces to the accurate Percus-Yevick equation for a hard core field. This and related equations give accurate results for a wide variety of fields